Nagakura K, Tachibana H, Kaneda Y, Nakae T
Exp Parasitol. 1986 Jun;61(3):335-42. doi: 10.1016/0014-4894(86)90188-8.
Two species of glycoproteins from Leishmania braziliensis promastigotes of apparent molecular weights of 53,000 (glycoprotein 53) and 47,000 (glycoprotein 47) were localized. Four lectins with different sugar specificities bound to the blotting sheet to which the electrophoretically separated materials were transferred. Concanavalin A and Ricinus communis agglutinin bound to the band of glycoprotein 53 and the lectin from Dolichos biflorus bound to the band of glycoprotein 47. Wheat germ agglutinin bound to the bands of both glycoproteins. Histochemical examinations using fluorescence labeled lectins demonstrated that the glycoproteins 53 and 47 were located on the cell surface and in the cytoplasm of promastigotes, respectively. The results are consistent with the result of agglutination test.
定位了来自巴西利什曼原虫前鞭毛体的两种糖蛋白，其表观分子量分别为53,000（糖蛋白53）和47,000（糖蛋白47）。四种具有不同糖特异性的凝集素与电泳分离材料转移至其上的印迹膜结合。伴刀豆球蛋白A和蓖麻凝集素与糖蛋白53条带结合，来自双花扁豆的凝集素与糖蛋白47条带结合。麦胚凝集素与两种糖蛋白条带均结合。使用荧光标记凝集素的组织化学检查表明，糖蛋白53和47分别位于前鞭毛体的细胞表面和细胞质中。结果与凝集试验结果一致。
