Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Khlong Luang, Pathumthani, Thailand.
Nefrologia. 2013 Jan 18;33(1):116-23. doi: 10.3265/Nefrologia.pre2012.Nov.11736.
Contrast-induced nephropathy (CIN) is a prominent cause of in-hospital acute kidney injury occurring after the administration of intravenous radiocontrast medium. Oxidative stress has been proposed as one of the more important mechanisms in the pathogenesis of CIN. The aim of the present study has been to determine the effect of alpha tocopherol on the reduction of renal damage in a rat model of CIN.
Male Sprague Dawley rats were subjected into six groups pretreated with alpha-tocopherol (250 or 500 mg/kg/day) or the vehicle tweeen80 for 5 days before the induction of CIN. Renal function and oxidative stress markers; level of malondialdehyde (MDA), total antioxidant capacity (TAC), superoxide dismutase (SOD) activity were determined. Kidney tissues were sectioned for pathohistological examination.
In the contrast media (CM) group, an increase in serum urea and creatinine was found. Tubular necrosis and peritubular capillary congestion were demonstrated in this group. Also, an imbalance of oxidative stress markers; an increase in MDA and a decreased SOD activity in kidney were shown. On the contrary, in CIN-induced rats administrated with alpha-tocopherol group, a significant reduction of renal function and renal MDA, together with a significant increase of renal SOD, were observed. Interestingly, a reduction in MDA and an increase of TAC in serum, along with prevention of tubular injury, were demonstrated in this group, as compared to the CM group.
This present study demonstrated that alpha tocopherol showed protective effect on the rat renal damage induced CIN. Therefore, this vitamin could be used as an antioxidant to attenuate the radiocontrast oxidative damage.
对比剂肾病（CIN）是静脉内应用造影剂后院内急性肾损伤的一个主要原因。氧化应激被认为是 CIN 发病机制中的一个重要机制。本研究旨在确定α-生育酚对 CIN 大鼠模型肾损伤减少的影响。
雄性 Sprague Dawley 大鼠用α-生育酚（250 或 500mg/kg/天）或赋形剂吐温 80 预处理 5 天，然后诱导 CIN。测定肾功能和氧化应激标志物；丙二醛（MDA）、总抗氧化能力（TAC）、超氧化物歧化酶（SOD）活性水平。肾组织切片行病理组织学检查。
在造影剂（CM）组中，发现血清尿素和肌酐升高。该组显示肾小管坏死和肾小管周围毛细血管充血。此外，氧化应激标志物失衡；肾 MDA 增加，SOD 活性降低。相反，在给予α-生育酚的 CIN 诱导大鼠组中，观察到肾功能和肾 MDA 显著降低，肾 SOD 显著增加。有趣的是，与 CM 组相比，该组血清 MDA 减少，TAC 增加，同时预防了肾小管损伤。
本研究表明，α-生育酚对 CIN 诱导的大鼠肾损伤具有保护作用。因此，这种维生素可用作抗氧化剂来减轻放射造影剂的氧化损伤。
