Department of Pathology, School of Medicine, Autonomous University of Nuevo Leon, Ave. Madero y Dr. Eduardo Aguirre P., Colonia Mitras Centro, Monterrey, Nuevo Leon C.P. 64460, Mexico.
Cell Biol Int. 2013 Mar;37(3):239-41. doi: 10.1002/cbin.10031. Epub 2013 Jan 30.
Healthy adult cartilage is thought to have little or no capacity to renewal, and cell turnover has not been reported in lung cartilage. We report that chondrocyte turnover occurs in lung cartilage, found in an unrelated study. Lung specimens from CD1 mice of 2, 6, 12, 18 or 24 months were fixed in 10% neutral-buffered formalin and paraffin-embedded. Apoptosis was analysed by in situ end-labelling of fragmented DNA. Proliferating cell nuclear antigen (PCNA) and nestin were examined by immunohistochemistry. Apoptosis and PCNA were detected in lung chondrocytes. Serial section analysis showed that cells in apoptosis were different from PCNA-positive cells, indicating that turnover was occurring. Chondrocytes were negative for nestin. Nestin-positive cells were present in connective tissue associated with cartilage, in some specimens in close proximity of it and in perivascular cells. Thus cell turnover in lung cartilage is possible, which may be mediated by nestin-positive cells.
健康的成年软骨被认为几乎没有或没有更新能力，肺软骨中也没有报道细胞更新。我们报告了在一项无关研究中发现的肺软骨中存在软骨细胞更新。将 2、6、12、18 或 24 个月的 CD1 小鼠的肺标本用 10%中性缓冲福尔马林固定并石蜡包埋。通过原位末端标记法分析 DNA 片段化来分析细胞凋亡。通过免疫组织化学检测增殖细胞核抗原 (PCNA) 和巢蛋白。在肺软骨细胞中检测到细胞凋亡和 PCNA。连续切片分析表明，凋亡细胞与 PCNA 阳性细胞不同，表明正在发生更新。软骨细胞巢蛋白呈阴性。巢蛋白阳性细胞存在于与软骨相关的结缔组织中，在一些标本中与软骨紧邻，也存在于血管周围细胞中。因此，肺软骨中的细胞更新是可能的，可能由巢蛋白阳性细胞介导。
