Department of Chemistry, University of Coimbra, 3004-535 Coimbra, Portugal.
Macromol Rapid Commun. 2013 May 14;34(9):717-22. doi: 10.1002/marc.201200734. Epub 2013 Jan 31.
The cationic, all-conjugated AB diblock copolymer poly[9,9-bis(2-ethylhexyl)fluorene]-b-poly[3-(6-trimethylammoniumhexyl) thiophene] bromide (PF2/6-b-P3TMAHT) shows dual fluorescence from the poly(fluorene) (PF) and poly(thiophene) (PT) blocks. A comparison of fluorescence quenching of the cationic PT block fluorescence with unquenched PF block provides a sensitive ratiometric method for anion sensing. The application to analysis of halide ions, single- and double-stranded DNA is demonstrated. High selectivity is observed with halide ions, with the strongest quenching being seen with iodide. The quenching with DNA can be used for nucleic acid quantification at sub-μM concentrations.
阳离子、全共轭 AB 嵌段共聚物聚[9,9-双(2-乙基己基)芴]-b-聚[3-(6-三甲基铵己基)噻吩]溴化物（PF2/6-b-P3TMAHT）显示出聚芴（PF）和聚噻吩（PT）嵌段的双重荧光。与未猝灭的 PF 块荧光相比，阳离子 PT 块荧光的荧光猝灭比较提供了一种用于阴离子感应的灵敏比率方法。该方法已应用于卤化物离子、单链和双链 DNA 的分析。观察到卤化物离子具有高选择性，其中碘化物的猝灭最强。与 DNA 的猝灭可用于亚微摩尔浓度下的核酸定量。
