Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
Evid Based Complement Alternat Med. 2012;2012:404012. doi: 10.1155/2012/404012. Epub 2012 Dec 26.
Polygonum chinense is a Malaysian ethnic plant with various healing effects. This study was to determine preventive effect of aqueous leaf extract of P. chinense against ethanol-induced gastric mucosal injury in rats. Sprague Dawley rats were divided into seven groups. The normal and ulcer control groups were orally administered with distilled water. The reference group was orally administered with 20 mg/kg omeprazole. The experimental groups received the extracts 62.5, 125, 250, and 500 mg/kg, accordingly. After sixty minutes, distilled water and absolute ethanol were given (5 mL/kg) to the normal control and the others, respectively. In addition to histology, immunohistochemical and periodic acid schiff (PAS) stains, levels of lipid peroxidation, malondialdehyde (MDA), antioxidant enzymes, and superoxide dismutase (SOD) were measured. The ulcer group exhibited severe mucosal damages. The experimental groups significantly reduced gastric lesions and MDA levels and increased SOD level. Immunohistochemistry of the experimental groups showed upregulation and downregulation of Hsp70 and Bax proteins, respectively. PAS staining in these groups exhibited intense staining as compared to the ulcer group. Acute toxicity study revealed the nontoxic nature of the extract. Our data provide first evidence that P. chinense extract could significantly prevent gastric ulcer.
菝葜是一种马来西亚民族植物，具有多种治疗效果。本研究旨在确定菝葜叶水提物对乙醇诱导的大鼠胃黏膜损伤的预防作用。将 Sprague Dawley 大鼠分为七组。正常组和溃疡对照组给予蒸馏水，参考组给予 20mg/kg 奥美拉唑。实验组分别给予 62.5、125、250 和 500mg/kg 的提取物。60 分钟后，正常对照组和其他组分别给予蒸馏水和无水乙醇（5mL/kg）。除了组织学、免疫组织化学和过碘酸希夫（PAS）染色外，还测量了脂质过氧化、丙二醛（MDA）、抗氧化酶和超氧化物歧化酶（SOD）的水平。溃疡组表现出严重的黏膜损伤。实验组显著减少胃损伤和 MDA 水平，并增加 SOD 水平。实验组的免疫组织化学显示 Hsp70 和 Bax 蛋白分别上调和下调。与溃疡组相比，这些组的 PAS 染色显示出强烈的染色。急性毒性研究表明提取物无毒。我们的数据首次提供了菝葜提取物可显著预防胃溃疡的证据。
