Nejati Alireza, Unsworth Charles P, Graham Euan S
Department of Engineering Science, University of Auckland, Auckland 1010, New Zealand.
Annu Int Conf IEEE Eng Med Biol Soc. 2012;2012:5368-71. doi: 10.1109/EMBC.2012.6347207.
Previously we developed an active contour method for segmenting and tracking cells in phase-contrast microscopy images. Our method is capable of fine-grained segmentation on noisy image sequences. In this paper, we improve the active contour segmentation model to provide better accuracy, by selectively identifying areas of the contour with low confidence and removing them. The method is applied to HMEC-1 cells (human microvascular endothelial cells). The segmentation provided by the method is quantitavely compared with manually-drawn contours, showing close fit and capability to 'lock' on to cell boundaries for hundreds of frames.
