Rachkov A E, Shul'ga N I, Starodub N F, El'skaia A V
Ukr Biokhim Zh (1978). 1990 Jan-Feb;62(1):17-24.
The homo- and heterologous tRNA were studied for their influence on the synthesis rate of globin and ovalbumin under simultaneous translation of their mRNAs in the tRNA-dependent cell-free system from wheat germ. In the presence of the fixed concentrations of one of the mRNAs the different amounts of the other mRNA (from 6 up to saturation of the system) were added to the samples. The ratio of the synthesis rate of the translation products after their electrophoretic characterization is determined on the basis of the autoradiogram scanning. The competition among mRNAs in question for some components of translation apparatus has been found: globin mRNA has a higher competitiveness than mRNA for ovalbumin. Modulation of the simultaneous translation of these mRNAs has been shown to depend on the nature of tRNA. The presence of homologous tRNA in the system increases a competitiveness of the corresponding mRNA, but heterologous tRNA reduces it. This regularity is retained in a wide range of the concentration ratios for the both mRNAs.
在从小麦胚芽中提取的依赖于tRNA的无细胞系统中，同时翻译珠蛋白和卵清蛋白的mRNA时，研究了同源和异源tRNA对它们合成速率的影响。在一种mRNA固定浓度存在的情况下，向样品中添加不同量的另一种mRNA（从6到系统饱和）。根据放射自显影片扫描确定翻译产物经电泳表征后的合成速率之比。已发现所讨论的mRNA之间对翻译装置某些成分存在竞争：珠蛋白mRNA比卵清蛋白mRNA具有更高的竞争力。已表明这些mRNA同时翻译的调节取决于tRNA的性质。系统中同源tRNA的存在增加了相应mRNA的竞争力，但异源tRNA则降低了它的竞争力。在两种mRNA的广泛浓度比范围内，这种规律都得以保持。
