Le Meur M A, Gerlinger P, Ebel J P
Eur J Biochem. 1976 Aug 16;67(2):519-26. doi: 10.1111/j.1432-1033.1976.tb10718.x.
The effect of tRNA distribution in the cell on the rate of specific protein synthesis has been investigated. A tRNA-dependent cell-free protein-synthesizing system derived from Krebs-II ascites cells has been worked out. In this system it is possible to synthesize specific proteins (egg-white proteins or globin) by the translation of oviduct or reticulocyte messenger RNA in the presence of tRNA from homologous or heterologous tissues. The rate of translation of a give messenger RNA is optimal in the presence of tRNA from the homologous tissue. The lower level of protein synthesis obtained with an excess of heterologous tRNA can be overcome by adding the homologous tRNA. Nevertheless homologous isoaccepting species partly purified by a reversed-phase chromatography or a benzoylated DEAE-cellulose column cannot fully restore the optimal synthesis, eliminating the hypothesis according to which a particular tRNA species is involved in this phenomenon. The correct distribution of tRNA is necessary for optimal translation of a messenger RNA.
已对细胞中tRNA分布对特定蛋白质合成速率的影响进行了研究。已建立了一种源自克雷布斯II腹水细胞的依赖tRNA的无细胞蛋白质合成系统。在该系统中，在来自同源或异源组织的tRNA存在下，通过输卵管或网织红细胞信使RNA的翻译，可以合成特定蛋白质（蛋清蛋白或珠蛋白）。在来自同源组织的tRNA存在下，给定信使RNA的翻译速率最佳。通过添加同源tRNA可以克服过量异源tRNA导致的蛋白质合成水平降低的问题。然而，通过反相色谱法或苯甲酰化DEAE-纤维素柱部分纯化的同源同功受体种类不能完全恢复最佳合成，从而排除了特定tRNA种类参与此现象的假设。tRNA的正确分布对于信使RNA的最佳翻译是必要的。
