Identification of precipitable Ca2+ by electron spectroscopic imaging and electron energy loss spectroscopy in the organ of Corti of the guinea pig.

Ca2+ cations were precipitated with potassium antimonate in the cochlea of the guinea pig, and the formed precipitates were localized by electron microscopy using either elastically or inelastically scattered electrons. The elemental composition of the precipitates was determined by electron spectroscopic imaging (ESI) and electron energy loss spectroscopy (EELS). It was found that calcium, antimony and oxygen were the dominating elements in the precipitates formed in different cell types in the inner ear.