Department of Orthodontics, University of Ege, Izmir, Turkey.
Angle Orthod. 2013 Sep;83(5):858-63. doi: 10.2319/102312-827.1. Epub 2013 Jan 31.
To test the null hypothesis that combining low-viscosity caries infiltrant with conventional adhesive resins would not improve sealing of sound enamel against demineralization in vitro.
Bovine enamel discs (N = 60) with diameter of 3 mm were randomly assigned to six groups (n = 10). The discs were etched with 37% phosphoric acid for 30 seconds and treated with resins of different monomer content forming the following groups: (1) Icon (DMG), (2) Transbond XT Primer (3M ESPE), (3) Heliobond (Ivoclar Vivadent), (4) Icon + Transbond XT Primer, and (5) Icon + Heliobond. Untreated etched samples served as the negative control. Specimens were subjected to demineralization by immersion in hydrochloric acid (pH 2.6) for 80 hours. Calcium dissolution into the acid was assessed by colorimetric analysis using Arsenazo III method at 16-hour intervals. Groups presenting high protection against demineralization were subjected to further acidic challenge for 15 days with calcium measurements repeated at 24-hour intervals. Data were analyzed by Kruskal-Wallis test and Mann-Whitney U-test.
Untreated specimens showed the highest amount of demineralization. Icon and Transbond XT primer decreased the mineral loss significantly compared to the control. Heliobond performed significantly better than both Icon and Transbond XT primer. Combination of Icon both with Transbond XT primer or Heliobond served as the best protective measures and maintained the protective effect for the additional 15-day acidic challenge.
Within the limitations of this in vitro study, it could be concluded that the use of low-viscosity caries infiltrant prior to application of the tested conventional adhesives increases their protective effect against demineralization.
验证以下无效假设，即将低黏度龋蚀渗透剂与传统黏接树脂结合使用不会提高体外对脱矿的健康釉质的密封效果。
将直径为 3 毫米的牛牙釉质圆盘（N=60）随机分为 6 组（n=10）。用 37%磷酸酸蚀 30 秒，用不同单体含量的树脂处理，形成以下几组：（1）Icon（DMG），（2）Transbond XT Primer（3M ESPE），（3）Heliobond（Ivoclar Vivadent），（4）Icon+Transbond XT Primer，和（5）Icon+Heliobond。未处理的酸蚀样本作为阴性对照。将样本浸泡在盐酸（pH2.6）中 80 小时以进行脱矿。使用 Arsenazo III 法在 16 小时间隔通过比色分析评估钙溶解到酸中的情况。高抗脱矿组进一步进行 15 天的酸性挑战，每 24 小时重复进行钙测量。数据采用 Kruskal-Wallis 检验和 Mann-Whitney U 检验进行分析。
未处理的样本显示出最高的脱矿量。Icon 和 Transbond XT Primer 与对照组相比，明显减少了矿物质损失。Heliobond 的表现明显优于 Icon 和 Transbond XT Primer。Icon 与 Transbond XT Primer 或 Heliobond 的组合是最佳的保护措施，并在额外的 15 天酸性挑战中保持了保护效果。
在本体外研究的限制范围内，可以得出结论，在应用测试用传统黏接剂之前使用低黏度龋蚀渗透剂可以增加它们对脱矿的保护效果。
