IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, Leo-Brandt-Str. 1, 52425 Jülich, Germany.
Chem Commun (Camb). 2013 Mar 11;49(20):2061-3. doi: 10.1039/c3cc38607d.
We report the first rationally designed (S)-selective MenD from E. coli for the synthesis of functionalized α-hydroxy ketones. By mutation of two amino acids in the active site stereoselectivity of the (R)-selective EcMenD (ee > 93%) was inverted giving access to (S)-5-hydroxy-4-oxo-5-phenylpentanoate derivatives with stereoselectivities up to 97% ee.
我们报告了第一个从大肠杆菌中设计的（S）-选择性 MenD，用于合成功能化的α-羟基酮。通过突变活性位点中的两个氨基酸，将（R）-选择性 EcMenD（ee＞93％）的立体选择性反转，得到（S）-5-羟基-4-氧代-5-苯基戊酸酯衍生物，立体选择性高达 97％ee。
