Bailly C, Imbert-Teboul M, Roinel N, Amiel C
Laboratoire de Physiologie Rénale, Faculté Xavier Bichat, Université Paris, France.
Am J Physiol. 1990 May;258(5 Pt 2):F1224-31. doi: 10.1152/ajprenal.1990.258.5.F1224.
The effect of isoproterenol (Iso) on tubular transport in the thick ascending limb of Henle's loop (TAL) was investigated by in vitro microperfusion of MTAL (medullary) and CTAL (cortical) from White Swiss mouse kidney. The pattern of activation of adenylate cyclase along the distal tubule was investigated in this strain: results indicated that Iso stimulated adenylate cyclase fivefold in MTAL and ninefold in CTAL. Data from microperfusion experiments showed that Iso (10(-7) M in the bath) significantly and reversibly increased Ca and Mg reabsorption in CTAL. No net transport of Ca and Mg was observed in MTAL whether Iso was present or not. With regard to Na and Cl, Iso significantly stimulated their reabsorption in both segments and increased the transepithelial voltage in MTAL. Iso abolished K reabsorption in MTAL and induced a net K secretion in CTAL, the latter effect being also observed with 10(-9) M of Iso. When applied on CTAL, propranolol (10(-6) M in the bath) inhibited all these effects. These data indicate that beta-adrenergic agonists are involved in the multihormonal modulation of the TAL function.
通过对来自瑞士小白鼠肾脏的髓质厚升支（MTAL）和皮质厚升支（CTAL）进行体外微灌注，研究了异丙肾上腺素（Iso）对亨氏袢厚升支（TAL）肾小管转运的影响。在该品系中研究了沿远端小管的腺苷酸环化酶激活模式：结果表明，Iso使MTAL中的腺苷酸环化酶活性提高了5倍，使CTAL中的腺苷酸环化酶活性提高了9倍。微灌注实验数据显示，浴液中10⁻⁷ M的Iso显著且可逆地增加了CTAL中钙和镁的重吸收。无论是否存在Iso，MTAL中均未观察到钙和镁的净转运。关于钠和氯，Iso显著刺激了两个节段中它们的重吸收，并增加了MTAL中的跨上皮电压。Iso消除了MTAL中的钾重吸收，并在CTAL中诱导了净钾分泌，10⁻⁹ M的Iso也观察到了后一种效应。当应用于CTAL时，浴液中10⁻⁶ M的普萘洛尔抑制了所有这些效应。这些数据表明，β-肾上腺素能激动剂参与了TAL功能的多激素调节。
