铜绿假单胞菌的甘氨酸代谢：氰化氢生物合成
Glycine metabolism by Pseudomonas aeruginosa: hydrogen cyanide biosynthesis.
作者信息
Castric P A
出版信息
J Bacteriol. 1977 May;130(2):826-31. doi: 10.1128/jb.130.2.826-831.1977.
Abstract
Hydrogen cyanide (HCN) production by Pseudomonas aeruginosa in a synthetic medium is stimulated by the presence of glycine. Methionine enhances this stimulation but will not substitute for glycine as a stimulator of cyanogenesis. Threonine and phenylalanine are effective substitutes for glycine in the stimulation of HCN production. Glycine, threonine, and serine are good radioisotope precursors of HCN, but methionine and phenylalanine are not. Cell extracts of P. aeruginosa convert [14C]threonine to [14C]glycine. H14CN is produced with low dilution of label from either [1-14C]glycine or [2-14C]glycine, indicating a randomization of label either in the primary or secondary metabolism of glycine. When whole cells were fed [1,2-14C]glycine, cyanide and bicarbonate were the only radioactive extracellular products observed.
摘要
在合成培养基中，铜绿假单胞菌产生氰化氢（HCN）会受到甘氨酸的刺激。蛋氨酸会增强这种刺激作用，但不能替代甘氨酸作为氰化物生成的刺激物。苏氨酸和苯丙氨酸在刺激HCN产生方面是甘氨酸的有效替代物。甘氨酸、苏氨酸和丝氨酸是HCN的良好放射性同位素前体，但蛋氨酸和苯丙氨酸不是。铜绿假单胞菌的细胞提取物可将[14C]苏氨酸转化为[14C]甘氨酸。用[1-14C]甘氨酸或[2-14C]甘氨酸产生的H14CN的标记稀释度较低，这表明甘氨酸在初级或次级代谢中标记发生了随机化。当给完整细胞提供[1,2-14C]甘氨酸时，观察到氰化物和碳酸氢盐是仅有的放射性细胞外产物。
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