假单胞菌属中甘氨酸氧化形成氰化物
Cyanide formation from oxidation of glycine of Pseudomonas species.
作者信息
Wissing F
出版信息
J Bacteriol. 1974 Mar;117(3):1289-94. doi: 10.1128/jb.117.3.1289-1294.1974.
Abstract
With whole cells of a hydrogen cyanide-producing bacterium strain C, of the genus Pseudomonas, it was found that the oxygen necessary for the oxidation of glycine to cyanide could be replaced by various artificial electron acceptors. The order of reactivity was: oxygen > phenazine methosulphate > methylene blue > 2,6-dichlorophenolindophenol > ferricyanide. Cyanide production was inhibited by pyrrolnitrin, a well-known inhibitor of many flavine enzymes. The molar ratio of added glycine to cyanide produced was found to be 1.09. With whole bacteria the apparent K(m) (glycine) for the cyanide production was found to be 5.0 x 10(-4) M.
摘要
利用假单胞菌属中一株产氰化氢细菌菌株C的全细胞，发现甘氨酸氧化为氰化物所需的氧可被各种人工电子受体替代。反应活性顺序为：氧＞吩嗪硫酸甲酯＞亚甲基蓝＞2,6-二氯酚靛酚＞铁氰化物。氰化物的产生受到硝吡咯菌素的抑制，硝吡咯菌素是许多黄素酶的著名抑制剂。发现添加的甘氨酸与产生的氰化物的摩尔比为1.09。对于全细菌，发现产生氰化物的表观K(m)（甘氨酸）为5.0×10⁻⁴M。
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