Department of Cell and Molecular Biology, "Iuliu Hațieganu" University of Medicine and Pharmacy, 6 Pasteur St., 400349, Cluj-Napoca, Romania.
Microsc Microanal. 2013 Apr;19(2):393-405. doi: 10.1017/S1431927612014195. Epub 2013 Feb 4.
Ultrastructural answer of bone marrow erythroid series and of red blood cells (RBCs) in Wistar rats to bee venom (BV) was analyzed by transmission and scanning electron microscopy, and corroborated with hematological data. A 5-day and a 30-day treatment with daily doses of 700 μg BV/kg and an acute-lethal treatment with a single dose of 62 mg BV/kg were performed. The 5-day treatment resulted in a reduced cellularity of the bone marrow, with necrosed proerythroblasts, polymorphous erythroblasts, and reticulocytes with cytoplasmic extensions, and a lower number of larger RBCs, with poikilocytosis (acanthocytosis) and anisocytosis, and reduced concentrations of hemoglobin. After the 30-day treatment, the bone marrow architecture was restored, but polymorphous erythroblasts and reticulocytes with thin extensions could still be observed, while the RBCs in higher number were smaller, many with abnormal shapes, especially acanthocytes. The acute treatment produced a partial depopulation of the bone marrow and ultrastructural changes of erythroblasts including abnormal mitochondrial cristae. The RBCs in lower number were bigger and crenated, with reduced concentrations of hemoglobin. Overall, BV was able to promote stress erythropoiesis in a time- and dose-related manner, mitochondrial cristae modification being a critical factor involved in the toxicity of the BV high doses.
通过透射和扫描电子显微镜分析了蜜蜂毒液（BV）对 Wistar 大鼠骨髓红系和红细胞（RBC）的超微结构反应，并与血液学数据相佐证。进行了为期 5 天和 30 天的每天 700μg BV/kg 的剂量治疗以及单次 62mg BV/kg 的急性致死剂量治疗。5 天的治疗导致骨髓细胞减少，有坏死的原红细胞、多形性红细胞和有细胞质延伸的网织红细胞，以及数量较少的较大 RBC，出现异形性（棘红细胞症）和大小不均，并且血红蛋白浓度降低。30 天的治疗后，骨髓结构得到恢复，但仍可观察到多形性红细胞和有薄延伸的网织红细胞，而数量较多的 RBC 较小，许多形状异常，特别是棘红细胞症。急性治疗导致骨髓部分排空和红细胞的超微结构改变，包括异常的线粒体嵴。数量较少的 RBC 更大且呈锯齿状，血红蛋白浓度降低。总的来说，BV 能够以时间和剂量相关的方式促进应激性红细胞生成，线粒体嵴的改变是 BV 高剂量毒性的一个关键因素。
