Human marrow erythropoiesis in culture: III. Ultrastructural and cytochemical studies of cellular interactions.

Human erythroblasts cultured with a methylcellulose clonal assay technique were studied with ultrastructural and cytochemical methods. The intact colonies contained only erythroblasts at a similar stage of maturation, and no macrophages were identified within the colonies. The cultured erythroblasts demonstrated many of the morphologic features described in vivo. Neutral and acid glycoconjugates identified on the plasmalemma stained in a similar way to that seen in vivo with a concanavalin A horseradish peroxidase bridge and dialyzed iron technique. Weak acid phosphatase activity and ferritin-like particles were demonstrated in siderosomes, but these structures lacked peroxidase activity and dialyzed iron reactive acid mucosubstance. Transmission and scanning electron microscopy identified numerous processes which connected early erythroblasts and resembled those described in the marrow of patients with dyserythropoietic disorders. These findings suggest the presence of abnormalities in cultured erythrocytes which should be considered when evaluating pathologic specimens in vitro.