Parmley R T, Ogawa M, Spicer S S, Bank H L, Wright N J
Exp Hematol. 1978 Jan;6(1):78-90.
Human erythroblasts cultured with a methylcellulose clonal assay technique were studied with ultrastructural and cytochemical methods. The intact colonies contained only erythroblasts at a similar stage of maturation, and no macrophages were identified within the colonies. The cultured erythroblasts demonstrated many of the morphologic features described in vivo. Neutral and acid glycoconjugates identified on the plasmalemma stained in a similar way to that seen in vivo with a concanavalin A horseradish peroxidase bridge and dialyzed iron technique. Weak acid phosphatase activity and ferritin-like particles were demonstrated in siderosomes, but these structures lacked peroxidase activity and dialyzed iron reactive acid mucosubstance. Transmission and scanning electron microscopy identified numerous processes which connected early erythroblasts and resembled those described in the marrow of patients with dyserythropoietic disorders. These findings suggest the presence of abnormalities in cultured erythrocytes which should be considered when evaluating pathologic specimens in vitro.
采用甲基纤维素克隆分析技术培养的人成红细胞，运用超微结构和细胞化学方法进行了研究。完整的集落仅包含处于相似成熟阶段的成红细胞，集落内未识别出巨噬细胞。培养的成红细胞展现出许多体内描述的形态学特征。通过伴刀豆球蛋白A辣根过氧化物酶桥和透析铁技术，在质膜上鉴定出的中性和酸性糖缀合物，其染色方式与体内所见相似。在铁小体中显示出弱酸磷酸酶活性和类铁蛋白颗粒，但这些结构缺乏过氧化物酶活性和透析铁反应性酸性粘多糖物质。透射电子显微镜和扫描电子显微镜识别出众多连接早期成红细胞的突起，这些突起类似于骨髓发育异常患者骨髓中所描述的突起。这些发现提示培养的红细胞存在异常，在体外评估病理标本时应予以考虑。
