Department of Radiation Oncology, Cancer Hospital of Shantou University Medical College, 7 Raoping Road, Shantou, Guangdong, 515041, China.
Radiat Oncol. 2013 Feb 1;8:31. doi: 10.1186/1748-717X-8-31.
To evaluate the association between single nucleotide polymorphisms (SNPs) at the 194 and 399 codons of XRCC1, and the risk of severe acute skin and oral mucosa reactions in nasopharyngeal carcinoma patients in China.
114 patients with nasopharyngeal carcinoma were sequentially recruited in this study. Heparinized peripheral blood samples were taken for SNPs analysis before the start of radiation treatment. SNPs in XRCC1 (194Arg/Trp and 399Arg/Gln) gene were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Dermatitis at upper neck and oral mucositis were clinically recorded according to the Common Terminology Criteria for Adverse Events v.3.0.
The variant allele frequencies were 0.289 for XRCC1 194Trp and 0.263 for XRCC1 399Gln. Of the 114 patients, 24 experienced grade 3 acute dermatitis and 48 had grade 3 acute mucositis. The XRCC1 399Arg/Gln was significantly associated with the development of grade 3 dermatitis (Odds Ratio, 2.65; 95% CI, 1.04-6.73; p = 0.037, χ2 = 4.357). In addition, it was also associated with higher incidence of grade 3 mucositis with a borderline statistical significance (Odds Ratio, 2.11; 95% CI, 0.951-4.66; p = 0.065, χ2 = 3.411). The relationship between XRCC1 194Arg/Trp and acute dermatitis, and mucositis was not found.
Our investigation shows, for the first time, that patients with the XRCC1 399Arg/Gln genotype were more likely to experience severe acute dermatitis and oral mucositis. With further validation, the information can be used to determine personalized radiotherapy strategy.
评估中国鼻咽癌患者 XRCC1 第 194 和 399 密码子的单核苷酸多态性（SNPs）与严重急性皮肤和口腔黏膜反应风险之间的关联。
本研究连续招募了 114 例鼻咽癌患者。在开始放射治疗前，采集肝素化外周血样进行 SNPs 分析。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析 XRCC1（194Arg/Trp 和 399Arg/Gln）基因中的 SNPs。根据通用不良事件术语标准 v.3.0 临床记录上颈皮炎和口腔黏膜炎。
XRCC1 194Trp 的变异等位基因频率为 0.289，XRCC1 399Gln 的变异等位基因频率为 0.263。在 114 例患者中，24 例发生 3 级急性皮炎，48 例发生 3 级急性黏膜炎。XRCC1 399Arg/Gln 与 3 级皮炎的发生显著相关（优势比，2.65；95%可信区间，1.04-6.73；p = 0.037，χ2 = 4.357）。此外，它与 3 级黏膜炎的高发生率也有一定的统计学意义（优势比，2.11；95%可信区间，0.951-4.66；p = 0.065，χ2 = 3.411）。未发现 XRCC1 194Arg/Trp 与急性皮炎和黏膜炎之间的关系。
我们的研究首次表明，XRCC1 399Arg/Gln 基因型的患者更有可能发生严重的急性皮炎和口腔黏膜炎。进一步验证后，这些信息可用于确定个体化的放射治疗策略。
