Pôle de Recherche en Gynécologie, Institut de Recherche Expérimentale et Clinique, Université Catholique de Louvain, Brussels, Belgium.
Fertil Steril. 2013 Apr;99(5):1363-1368.e2. doi: 10.1016/j.fertnstert.2012.12.016. Epub 2013 Jan 29.
To evaluate the survival and growth potential of human preantral follicles isolated before and after cryopreservation.
Pilot study.
Gynecology research unit in a university hospital.
PATIENT(S): Six women aged 27 to 32 years.
INTERVENTION(S): Six ovarian biopsy samples were cut into two equal parts, half subjected to slow-freezing followed by follicle isolation (cryo-iso group) and alginate-matrigel embedding, and half immediately processed for follicle isolation and alginate-matrigel embedding followed by slow-freezing (iso-cryo group) or used as fresh controls (fresh group).
MAIN OUTCOME MEASURE(S): Follicle number, viability, diameter, and morphology.
RESULT(S): After 1,134 preantral follicles had been isolated from fresh biopsy samples and 1,132 from frozen specimens, the three groups were compared before and after 7 days of in vitro culture (IVC) in alginate-matrigel beads. No statistically significant differences in viability were found between the three groups before or after IVC, but follicle diameter increased in all three groups after IVC. Morphology analysis revealed well-preserved follicles in both the iso-cryo and cryo-iso groups after IVC.
CONCLUSION(S): Human preantral follicles can be successfully cryopreserved before or after isolation without impairing their ability to survive and grow in vitro. This could lead to development of new protocols for follicle cryopreservation, IVC, and grafting in clinical and research settings for fertility preservation.
评估在冷冻保存前后分离的人原始卵泡的存活和生长潜能。
初步研究。
大学医院的妇科研究单位。
6 名年龄在 27 至 32 岁的女性。
6 个卵巢活检样本被切成相等的两半，一半进行慢速冻处理，然后进行卵泡分离（冷冻-分离组）和藻酸盐-基质胶包埋，另一半立即进行卵泡分离和藻酸盐-基质胶包埋，然后进行慢速冻处理（分离-冷冻组）或作为新鲜对照（新鲜组）。
卵泡数量、活力、直径和形态。
在从新鲜活检样本中分离出 1134 个原始卵泡和从冷冻标本中分离出 1132 个原始卵泡后，比较了三组在体外培养（IVC）在藻酸盐-基质胶珠中 7 天后的情况。在 IVC 前后，三组之间的活力均无统计学差异，但所有三组的卵泡直径在 IVC 后均增加。形态学分析显示，在 IVC 后，分离-冷冻组和冷冻-分离组的卵泡均保存良好。
在不损害其在体外存活和生长能力的情况下，可以成功地在分离前后对人原始卵泡进行冷冻保存。这可能会导致在临床和研究环境中为生育力保存开发新的卵泡冷冻保存、IVC 和移植方案。
