Department of Laboratory, Indian Spinal Injuries Centre, Sector-C, Vasant Kunj, 110070 New Delhi, India.
J Mycol Med. 2013 Mar;23(1):21-6. doi: 10.1016/j.mycmed.2012.12.002. Epub 2013 Jan 30.
The aim of this study was to evaluate the rate of isolation and antifungal susceptibility profile of various opportunistic yeasts in faeces of patients with chronic diarrhea.
The study included 120 patients who received the consultation and treatment for the complaints of chronic diarrhea for a period of more than 15 days.
The microscopy of samples of stool was followed by the culture on Sabourauds-dextrose agar. Isolate were tried for their sensitivity to fluconazole, flucytosine, amphotericin B and voriconazole.
The significant growth of fungal organism was seen in 32 (26.7%) stool specimen. These fungal isolates include five (15.6%) of C. albicans, 14 (43.8%) of C. tropicalis, five (15.6%) of C. krusei, two (6.3%) of C. famata, two (6.3%) of C. parapsilosis isolates and one isolate each of C. lusitaniae, C. guilliermondii, Stephanoascus ciferri and Trichosporon asahii (3.1% each). Most of the yeast isolates (65.6%) were found to be sensitive to all four antifungal agents i.e., fluconazole, flucytosine, amphotericin B and voriconazole. The highest resistance was seen against fluconazole in 10 (31.3%) isolates followed by flucytosine in eight (25%) isolates. No resistance to voriconazole was observed in any of the isolates. The resistance to the antifungal agents was higher among the other species of Candida compared to C. albicans.
The study could establish the relation between the opportunistic yeasts and chronic diarrhea and gives information on the antifungal sensitivity profile of the isolated yeasts.
本研究旨在评估慢性腹泻患者粪便中各种机会性酵母菌的分离率和抗真菌药敏谱。
本研究纳入了 120 名因慢性腹泻症状持续超过 15 天就诊和治疗的患者。
对粪便样本进行显微镜检查，然后在沙氏葡萄糖琼脂上进行培养。对分离株进行氟康唑、氟胞嘧啶、两性霉素 B 和伏立康唑的药敏试验。
32 份（26.7%）粪便标本中真菌明显生长。这些真菌分离株包括 5 株（15.6%）白念珠菌、14 株（43.8%）热带念珠菌、5 株（15.6%）克柔念珠菌、2 株（6.3%）近平滑念珠菌、2 株（6.3%）葡萄牙念珠菌分离株，以及 1 株鲁氏毛孢子菌、1 株近平滑念珠菌、1 株斯蒂芬拟青霉和 1 株申克孢子丝菌（各占 3.1%）。大多数酵母分离株（65.6%）对所有四种抗真菌药物即氟康唑、氟胞嘧啶、两性霉素 B 和伏立康唑均敏感。对氟康唑的耐药性最高，有 10 株（31.3%）分离株，其次是氟胞嘧啶，有 8 株（25%）分离株。所有分离株均未对伏立康唑产生耐药性。与白念珠菌相比，其他念珠菌种对抗真菌药物的耐药性更高。
本研究确立了机会性酵母菌与慢性腹泻之间的关系，并提供了关于分离酵母菌抗真菌药敏谱的信息。
