利用生物膜形成菌固定 Rhodococcus rhodochrous BX2（一种可降解乙腈的细菌）处理废水。
Immobilization of Rhodococcus rhodochrous BX2 (an acetonitrile-degrading bacterium) with biofilm-forming bacteria for wastewater treatment.
机构信息
College of Resource and Environment, Northeast Agricultural University, Harbin 150030, Heilongjiang, PR China.
出版信息
Bioresour Technol. 2013 Mar;131:390-6. doi: 10.1016/j.biortech.2012.12.140. Epub 2012 Dec 31.
In this study, a unique biofilm consisting of three bacterial strains with high biofilm-forming capability (Bacillus subtilis E2, E3, and N4) and an acetonitrile-degrading bacterium (Rhodococcus rhodochrous BX2) was established for acetonitrile-containing wastewater treatment. The results indicated that this biofilm exhibited strong resistance to acetonitrile loading shock and displayed a typical spatial and structural heterogeneity and completely depleted the initial concentration of acetonitrile (800mgL(-1)) within 24h in a moving-bed-biofilm reactor (MBBR) after operation for 30days. The immobilization of BX2 cells in the biofilm was confirmed by PCR-DGGE. It has been demonstrated that biofilm-forming bacteria can promote the immobilization of contaminant-degrading bacteria in the biofilms and can subsequently improve the degradation of contaminants in wastewater. This approach offers a novel strategy for enhancing biological oxidation of toxic pollutants in wastewater.
在本研究中，建立了一种由三株具有高生物膜形成能力的细菌（枯草芽孢杆菌 E2、E3 和 N4）和一株乙腈降解菌（红球菌 rhodochrous BX2）组成的独特生物膜，用于处理含乙腈废水。结果表明，该生物膜对乙腈负荷冲击具有很强的抵抗力，表现出典型的空间和结构异质性，并且在运行 30 天后，在移动床生物膜反应器（MBBR）中，生物膜在 24 小时内完全耗尽初始浓度为 800mgL(-1)的乙腈。通过 PCR-DGGE 证实了 BX2 细胞在生物膜中的固定化。已经证明，生物膜形成细菌可以促进污染物降解细菌在生物膜中的固定化，从而可以提高废水中污染物的降解。该方法为增强废水中有毒污染物的生物氧化提供了一种新策略。
Zotero 插件