Department of Microbiology and Biotechnology Centre, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390002, India.
Department of Civil Engineering, Faculty of Technology and Engineering, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, 390001, India.
World J Microbiol Biotechnol. 2021 Mar 22;37(4):68. doi: 10.1007/s11274-021-03035-0.
Biological denitrification is the most promising alternative approach for the removal of nitrate from wastewater. MBBR inoculated with activated sludge is a widely studied approach, but very few studies have focused on the bioaugmentation of biofilm forming bacteria in MBBR. Our study revealed that the use of special microbial seed of biofilm forming denitrifying bacteria Diaphorobacter sp. R4, Pannonibacter sp. V5, Thauera sp. V9, Pseudomonas sp.V11, and Thauera sp.V14 to form biofilm on carriers enhanced nitrate removal performance of developed MBBR. Various process parameters C/N ratio 0.3, HRT 3 h at Nitrate loading 2400 mg L, Filling ratio 20%, operated with Pall ring carrier were optimized to achieve highest nitrate removal. After 300 days of continuous operation results of whole genome metagenomic studies showed that Thauera spp. were the most dominant and key contributor to the denitrification of nitrate containing wastewater and the reactor was totally conditioned for denitrification. Overall, findings suggest that bench-scale MBBR developed with biofilm forming denitrifying microbial seed accelerated the denitrification process; therefore in conclusion it is suggested as one of the best suitable and effective approach for removal of nitrate from wastewater.
生物反硝化是去除废水中硝酸盐最有前途的替代方法。接种活性污泥的 MBBR 是一种广泛研究的方法，但很少有研究关注 MBBR 中生物膜形成细菌的生物强化。我们的研究表明，使用特殊的生物膜形成脱氮菌 Diaphorobacter sp. R4、Pannonibacter sp. V5、Thauera sp. V9、Pseudomonas sp. V11 和 Thauera sp. V14 的微生物种作为种子接种在载体上，可以提高开发的 MBBR 的硝酸盐去除性能。优化了各种工艺参数，C/N 比为 0.3，HRT 为 3 小时，硝酸盐负荷为 2400 mg/L，填充率为 20%，采用鲍尔环载体，以达到最高的硝酸盐去除率。经过 300 天的连续运行，全基因组宏基因组研究结果表明，Thauera spp. 是含硝酸盐废水反硝化的最主要和关键贡献者，反应器完全适应了反硝化过程。总的来说，研究结果表明，采用生物膜形成脱氮微生物种开发的 MBBR 加速了反硝化过程；因此，建议将其作为去除废水中硝酸盐的最佳方法之一。
