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RdDM 通路是拟南芥基础耐热性所必需的。

The RdDM pathway is required for basal heat tolerance in Arabidopsis.

机构信息

GMI-Gregor Mendel Institute of Molecular Plant Biology, Austrian Academy of Sciences, Dr. Bohr-Gasse 3, 1030 Vienna, Austria.

出版信息

Mol Plant. 2013 Mar;6(2):396-410. doi: 10.1093/mp/sst023. Epub 2013 Feb 1.

DOI:10.1093/mp/sst023
PMID:23376771
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3603006/
Abstract

Heat stress affects epigenetic gene silencing in Arabidopsis. To test for a mechanistic involvement of epigenetic regulation in heat-stress responses, we analyzed the heat tolerance of mutants defective in DNA methylation, histone modifications, chromatin-remodeling, or siRNA-based silencing pathways. Plants deficient in NRPD2, the common second-largest subunit of RNA polymerases IV and V, and in the Rpd3-type histone deacetylase HDA6 were hypersensitive to heat exposure. Microarray analysis demonstrated that NRPD2 and HDA6 have independent roles in transcriptional reprogramming in response to temperature stress. The misexpression of protein-coding genes in nrpd2 mutants recovering from heat correlated with defective epigenetic regulation of adjacent transposon remnants which involved the loss of control of heat-stress-induced read-through transcription. We provide evidence that the transcriptional response to temperature stress, at least partially, relies on the integrity of the RNA-dependent DNA methylation pathway.

摘要

热应激影响拟南芥的表观遗传基因沉默。为了检验表观遗传调控在热应激反应中的机制作用，我们分析了在 DNA 甲基化、组蛋白修饰、染色质重塑或基于 siRNA 的沉默途径中存在缺陷的突变体的耐热性。缺乏 RNA 聚合酶 IV 和 V 的常见第二大亚基 NRPD2 和 Rpd3 型组蛋白去乙酰化酶 HDA6 的植物对热暴露敏感。微阵列分析表明，NRPD2 和 HDA6 在响应温度胁迫的转录重编程中具有独立的作用。在从热中恢复的 nrpd2 突变体中，蛋白质编码基因的错误表达与相邻转座子残余物的表观遗传调控缺陷相关，这涉及到对热应激诱导的通读转录的失控。我们提供的证据表明，对温度胁迫的转录反应至少部分依赖于 RNA 依赖性 DNA 甲基化途径的完整性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4df1/3603006/dab065d5404e/mpphys_sst023_f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4df1/3603006/1385a0f3e6b2/mpphys_sst023_f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4df1/3603006/5dff7301ebf3/mpphys_sst023_f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4df1/3603006/d5276c7359df/mpphys_sst023_f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4df1/3603006/acc508214bf1/mpphys_sst023_f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4df1/3603006/dab065d5404e/mpphys_sst023_f0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4df1/3603006/1385a0f3e6b2/mpphys_sst023_f0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4df1/3603006/5dff7301ebf3/mpphys_sst023_f0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4df1/3603006/d5276c7359df/mpphys_sst023_f0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4df1/3603006/acc508214bf1/mpphys_sst023_f0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4df1/3603006/dab065d5404e/mpphys_sst023_f0005.jpg

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3
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