School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, People's Republic of China.
Curr Microbiol. 2013 Jun;66(6):614-20. doi: 10.1007/s00284-013-0317-6. Epub 2013 Feb 3.
In this study, a lab-scale partial nitrifying sequencing batch reactor (SBR) was developed to investigate partial nitrification at ambient temperature (16-22 °C). Techniques of denaturing gradient gel electrophoresis (DGGE), cloning, and fluorescence in situ hybridization (FISH) were utilized simultaneously to study microbial population dynamics. Partial nitrification was effectively achieved in response to shifts of influent ammonium concentrations. DGGE results showed that higher ammonia concentration referred to lower ammonia-oxidizing bacteria (AOB) diversity in the SBR. Phylogenetic analysis revealed that all the predominant AOB was affiliated with Nitrosomonas genus. FISH analysis illustrated AOB was the predominant nitrifying bacteria of microbial compositions when SBR achieved partial nitrification (PN) at ambient temperature.
在这项研究中，开发了一种实验室规模的亚硝化序批式反应器（SBR），以研究环境温度（16-22°C）下的亚硝化作用。变性梯度凝胶电泳（DGGE）、克隆和荧光原位杂交（FISH）技术同时被用于研究微生物种群动态。亚硝化作用在进水氨氮浓度变化的情况下得到了有效实现。DGGE 结果表明，较高的氨氮浓度导致 SBR 中亚硝化菌（AOB）的多样性降低。系统发育分析表明，所有优势 AOB 都属于亚硝化单胞菌属。FISH 分析表明，当 SBR 在环境温度下实现部分硝化（PN）时，AOB 是微生物组成中的主要硝化细菌。
