State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai, 200092, P.R. China.
Engineering Research Center of Biomembrane Water Purification and Utilization Technology, Ministry of Education, School of Civil Engineering and Architecture, Anhui University of Technology, Ma'anshan, 243032, P.R. China.
Sci Rep. 2019 Feb 4;9(1):1153. doi: 10.1038/s41598-018-37841-8.
This study investigated the operation mode of a step-feed anoxic/oxic (A/O) process with distribution of the carbon source from the anaerobic zone in terms of the treatment effects on sewage with low carbon and high nitrogen and phosphorus. After seven phases of operation, an optimal flow distribution ratio of 75%:25% was obtained from the anaerobic zone, and the concentrations of chemical oxygen demand, ammonia nitrogen, total nitrogen, and total phosphorous in the effluent were 20.8, 0.64, 14.2, and 0.89 mg/L, respectively. The presence of an internal reflux system in the deaeration zone improved the treatment. 16S rRNA high-throughput sequencing revealed that the microbial communities in aerobic zone I(O1) of the first-step A/O sludge were different from those in aerobic zone I (O2) of the second-step A/O sludge, whereas microbial communities of the seed sludge were similar to those in O2 of the second-step A/O sludge. The richness and diversity of microbial communities in O1 of the first-step A/O sludge samples were higher than those in O2 of the second-step A/O and seed sludge. At the optimal flow distribution ratio, the microbial abundance and treatment removal efficiency were the highest.
本研究考察了分步缺氧/好氧（A/O）工艺的运行模式，即在低碳高氮磷污水处理方面，从厌氧区分配碳源。经过七轮运行，从厌氧区获得最佳的流量分配比为 75%:25%，出水的化学需氧量、氨氮、总氮和总磷浓度分别为 20.8、0.64、14.2 和 0.89mg/L。脱气区内部回流系统的存在提高了处理效果。16S rRNA 高通量测序表明，第一步 A/O 污泥好氧区 I（O1）的微生物群落与第二步 A/O 污泥好氧区 I（O2）的微生物群落不同，而种子污泥的微生物群落与第二步 A/O 污泥的 O2 中的微生物群落相似。第一步 A/O 污泥样品中 O1 的微生物群落丰富度和多样性均高于第二步 A/O 和种子污泥的 O2。在最佳流量分配比下，微生物丰度和处理去除效率最高。
