Barnes J M, Barnes N M, Costall B, Horovitz Z P, Ironside J W, Naylor R J, Williams T J
Postgraduate Studies in Pharmacology, School of Pharmacy, University of Bradford, U.K.
Brain Res. 1990 Jan 22;507(2):341-3. doi: 10.1016/0006-8993(90)90294-l.
Angiotensin II was shown to inhibit potassium-stimulated release of [3H]acetylcholine from slices of fresh human temporal cortex, obtained at surgery, and subsequently loaded with [3H]choline for the biochemical analyses. The inhibitory effect of angiotensin II was antagonised by the specific angiotensin II receptor antagonist [1-sarcosine, 8-threonine]-angiotensin II. High affinity binding sites were identified in the human temporal cortex using [125I]angiotensin II, and may provide the functional site of action of angiotensin II to modify [3H]acetylcholine release.
