促肾上腺皮质激素对针鼹（短吻针鼹）肾上腺类固醇生成及血液皮质类固醇水平的影响

The effects of ACTH on adrenal steroidogenesis and blood corticosteroid levels in the echidna (Tachyglossus aculeatus).

Weiss M, Oddie C J, McCance I

Department of Physiology, Monash University, Clayton, Victoria, Australia.

Comp Biochem Physiol B. 1979;64(1):65-70. doi: 10.1016/0305-0491(79)90184-6.

Abstract

The effects of short-term (S.T., 30 min) and long-term (L.T., 4 days) administration of ACTH on peripheral blood corticosteroid levels and on in vitro steroidogenesis were investigated. 2. Control levels of cortisol, corticosterone and aldosterone were 58 +/- 12, 130 +/- 26 and 10 +/- 6 (SEM) ng/100 ml respectively. 3. Corticosterone was 70% higher after S.T. and 150% higher after L.T., when cortisol was 800% higher. 4. Adrenal homogenates from control echidnas converted [14C]progesterone predominantly to 11-deoxycorticosterone (45%) and 11-deoxycortisol (12%). 5. After L.T. the principal product was corticosterone (25%), but S.T. had no effect. 6. In control echidnas the Km and V for 11 beta-hydroxylation of 11-deoxycorticosterone were 20 microM and 2.8 rho mol/min/mg respectively. After L.T. V increased to 10 rho mol/min/mg.

摘要

研究了促肾上腺皮质激素短期（30分钟）和长期（4天）给药对外周血皮质类固醇水平及体外类固醇生成的影响。2. 皮质醇、皮质酮和醛固酮的对照水平分别为58±12、130±26和10±6（标准误）纳克/100毫升。3. 短期给药后皮质酮升高70%，长期给药后升高150%，而皮质醇升高800%。4. 对照针鼹的肾上腺匀浆将[14C]孕酮主要转化为11-脱氧皮质酮（45%）和11-脱氧皮质醇（12%）。5. 长期给药后主要产物为皮质酮（25%），但短期给药无影响。6. 在对照针鼹中，11-脱氧皮质酮11β-羟化的米氏常数（Km）和最大反应速度（V）分别为20微摩尔和2.8纳摩尔/分钟/毫克。长期给药后V增加到10纳摩尔/分钟/毫克。