ARC Centre of Excellence for Electromaterials Science and Intelligent Polymer Research Institute, University of Wollongong, Wollongong, NSW 2522, Australia.
J Control Release. 2013 Aug 10;169(3):313-20. doi: 10.1016/j.jconrel.2013.01.022. Epub 2013 Feb 1.
We hereby present a new method of producing coaxial conducting polymer fibres loaded with an antibiotic drug that can then be subsequently released (or sustained) in response to electrical stimulation. The method involves wet-spinning of poly(3,4-ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS) fibre, which served as the inner core to the electropolymerised outer shell layer of polypyrrole (Ppy). Ciprofloxacin hydrochloride (Cipro) was selected as the model drug and as the dopant in the Ppy synthesis. The release of Cipro in phosphate buffered saline (PBS) from the fibres was controlled by switching the redox state of Ppy.Cipro layer. Released Cipro under passive and stimulated conditions were tested against Gram positive (Streptococcus pyogenes) and Gram negative (Escherichia coli) bacteria. Significant inhibition of bacterial growth was observed against both strains tested. These results confirm that Cipro retains antibacterial properties during fibre fabrication and electrochemically controlled release. In vitro cytotoxicity testing utilising the neural B35 cell line confirmed the cytocompatibility of the drug loaded conducting fibres. Electrical conductivity, cytocompatibility and tuning release profile from this flexible fibre can lead to promising bionic applications such as neuroprosthetics and localised drug delivery.
我们在此提出了一种生产载有抗生素药物的同轴导电聚合物纤维的新方法，这种纤维可以在受到电刺激时（或持续地）释放药物。该方法涉及聚（3,4-亚乙基二氧噻吩）聚（苯乙烯磺酸盐）（PEDOT：PSS）纤维的湿法纺丝，该纤维作为电聚合的聚吡咯（Ppy）外壳层的内芯。盐酸环丙沙星（Cipro）被选为模型药物，并作为 Ppy 合成中的掺杂剂。纤维中 Cipro 在磷酸盐缓冲盐水（PBS）中的释放通过切换 Ppy.Cipro 层的氧化还原状态来控制。在被动和刺激条件下释放的 Cipro 针对革兰氏阳性（化脓性链球菌）和革兰氏阴性（大肠杆菌）细菌进行了测试。对两种测试菌株均观察到显著的抑菌生长。这些结果证实，Cipro 在纤维制造和电化学控制释放过程中保持了抗菌性能。利用神经 B35 细胞系进行的体外细胞毒性测试证实了载药导电纤维的细胞相容性。这种柔性纤维的电导率、细胞相容性和释放曲线的调节可以为神经假体和局部药物输送等仿生应用提供有前途的解决方案。
