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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Dragos S, Radu M, Anca N

Department of Cell Biology, Faculty of Biology, Bucharest, Rumania.

Comp Biochem Physiol B. 1979;62(4):335-8. doi: 10.1016/0305-0491(79)90099-3.

Concentration of NAD during embryonic development of haploid and diploid embryos of frog was followed. NAD content in haploid embryonic forms is twice that in diploid embryos. 2. The variation of the NMN adenylyltransferase activity in the oocytes and during the first states of embryonic development as surveyed in the nuclear soluble fraction and the nuclear insoluble fraction (chromatin). 3. The enzyme activity in the soluble fraction is low during embryonic development and shows higher values in haploid embryos. 4. In the nonfertilized mature oocytes, the NMN adenylyltransferase activity is sixfold higher in the insoluble chromatin fraction than in the soluble fraction. 5. The evolution of the NMN adenylyltransferase in the insoluble chromatin fraction also shows higher values in haploid embryos, as compared with diploid forms.

对青蛙单倍体和二倍体胚胎发育过程中NAD的浓度进行了跟踪。单倍体胚胎形式中的NAD含量是二倍体胚胎的两倍。2. 对卵母细胞以及胚胎发育最初阶段核可溶性部分和核不溶性部分（染色质）中NMN腺苷酸转移酶活性的变化进行了研究。3. 可溶性部分中的酶活性在胚胎发育过程中较低，在单倍体胚胎中显示出较高的值。4. 在未受精的成熟卵母细胞中，不溶性染色质部分的NMN腺苷酸转移酶活性比可溶性部分高六倍。5. 与二倍体形式相比，不溶性染色质部分中NMN腺苷酸转移酶在单倍体胚胎中的演变也显示出较高的值。

Dragos S, Radu M, Anca N

Department of Cell Biology, Faculty of Biology, Bucharest, Rumania.

Comp Biochem Physiol B. 1979;62(4):335-8. doi: 10.1016/0305-0491(79)90099-3.

Concentration of NAD during embryonic development of haploid and diploid embryos of frog was followed. NAD content in haploid embryonic forms is twice that in diploid embryos. 2. The variation of the NMN adenylyltransferase activity in the oocytes and during the first states of embryonic development as surveyed in the nuclear soluble fraction and the nuclear insoluble fraction (chromatin). 3. The enzyme activity in the soluble fraction is low during embryonic development and shows higher values in haploid embryos. 4. In the nonfertilized mature oocytes, the NMN adenylyltransferase activity is sixfold higher in the insoluble chromatin fraction than in the soluble fraction. 5. The evolution of the NMN adenylyltransferase in the insoluble chromatin fraction also shows higher values in haploid embryos, as compared with diploid forms.

对青蛙单倍体和二倍体胚胎发育过程中NAD的浓度进行了跟踪。单倍体胚胎形式中的NAD含量是二倍体胚胎的两倍。2. 对卵母细胞以及胚胎发育最初阶段核可溶性部分和核不溶性部分（染色质）中NMN腺苷酸转移酶活性的变化进行了研究。3. 可溶性部分中的酶活性在胚胎发育过程中较低，在单倍体胚胎中显示出较高的值。4. 在未受精的成熟卵母细胞中，不溶性染色质部分的NMN腺苷酸转移酶活性比可溶性部分高六倍。5. 与二倍体形式相比，不溶性染色质部分中NMN腺苷酸转移酶在单倍体胚胎中的演变也显示出较高的值。