Laboratory of Morphometry, Metabolism and Cardiovascular Disease, Biomedical Centre, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Rio de Janeiro, Brazil.
PLoS One. 2013;8(1):e55711. doi: 10.1371/journal.pone.0055711. Epub 2013 Jan 31.
Maternal obesity induced by a high fat (HF) diet may program susceptibility in offspring, altering pancreatic development and causing later development of chronic degenerative diseases, such as obesity and diabetes. Female mice were fed standard chow (SC) or an HF diet for 8 weeks prior to mating and during the gestational and lactational periods. The male offspring were assessed at birth, at 10 days, and at 3 months of age. The body mass (BM) gain was 50% greater before pregnancy and 80% greater during pregnancy in HF dams than SC dams. Dams fed an HF diet showed higher oral glucose tolerance test (OGTT), blood pressure, serum corticosterone, and insulin levels than dams fed SC. At 10 days of age and at 3 mo old the HF offspring showed greater BM and higher blood glucose levels than the SC offspring. The mean diameter of the islets had increased by 37% in the SC offspring and by 155% in the HF offspring at 10 days of age. The islet mass ratio (IM/PM) was 88% greater in the HF offspring at 10 days of age, and 107% greater at 3 mo of age, compared to the values obtained at birth. The HF offspring had a beta cell mass (BCM)/PM ratio 54% lower than SC offspring at birth. However, HF offspring displayed a 146% increase in the BCM/PM ratio at 10 days of age, and 112% increase at 3 months of age than values at birth. A 3 mo of age, the HF offspring showed a greater OGTT and higher levels of than SC offspring. In conclusion, a maternal HF diet consumed during the preconceptional period and throughout the gestational and lactational periods in mice results in dramatic alterations in the pancreata of the offspring.
高脂肪（HF）饮食引起的母体肥胖可能会使后代易感性增加，改变胰腺发育，并导致肥胖和糖尿病等慢性退行性疾病的后期发展。雌性小鼠在交配前、妊娠期和哺乳期接受标准饮食（SC）或高脂肪饮食（HF）喂养 8 周。雄性后代在出生时、10 天时和 3 月龄时进行评估。HF 组母鼠的体质量（BM）在怀孕前增加了 50%，在怀孕期间增加了 80%，而 SC 组母鼠则没有。与 SC 组相比，HF 组母鼠的口服葡萄糖耐量试验（OGTT）、血压、血清皮质酮和胰岛素水平更高。在 10 天和 3 月龄时，HF 组后代的 BM 更大，血糖水平更高。10 天时，SC 组后代的胰岛平均直径增加了 37%，HF 组增加了 155%。10 天时，HF 组后代的胰岛质量比（IM/PM）比出生时增加了 88%，3 月龄时增加了 107%。与 SC 组相比，HF 组后代出生时的β细胞质量（BCM）/PM 比值低 54%。然而，HF 组后代在 10 天时的 BCM/PM 比值增加了 146%，3 个月时增加了 112%，高于出生时的水平。3 月龄时，HF 组后代的 OGTT 和血糖水平高于 SC 组后代。总之，在小鼠中，在受孕前和整个妊娠期及哺乳期摄入 HF 饮食会导致后代胰腺发生显著变化。
