Suzuki N, Shirako Y, Ehara Y
Laboratory of Plant Genetic Engineering, Akita Prefectural College of Agriculture, Japan.
Intervirology. 1990;31(1):43-9. doi: 10.1159/000150133.
Cylindrical inclusion proteins (CIPs), amorphous inclusion proteins (AIPs), and virus particles were partially purified from Cucurbita maxima leaves infected with zucchini yellow mosaic virus (ZYMV), watermelon mosaic virus 2 (WMV2), and the watermelon mosaic virus 1 strain of papaya ringspot virus (PRSV-W). Antisera to these individual antigens, except AIPs of ZYMV and WMV2, were prepared and used to determine with electroblot-ELISA the serological differentiation indices (SDIs) between homologous and heterologous antigens. The average SDIs between ZYMV and WMV2 CIPs, between ZYMV and PRSV-W CIPs, and between WMV2 and PRSV-W CIPs were 1.5, 3.5, and 2.5, respectively. The SDIs between ZYMV and PRSV-W AIPs and between WMV2 and PRSV-W AIPs were 5 and 3, respectively. The average SDIs between ZYMV and WMV2 capsid proteins (CPs), between ZYMV and PRSV-W CPs, and between WMV2 and PRSV-W CPs were 3, 7, and 8, respectively. These data suggest that the serological relationship between ZYMV and WMV2 is much closer than that between PRSV-W and ZYMV or between PRSV-W and WMV2 and that antigenic determinants of CIP and AIP were conserved more than those of CP among the three potyviruses.
从感染西葫芦黄花叶病毒（ZYMV）、西瓜花叶病毒2（WMV2）和木瓜环斑病毒西瓜花叶病毒1株系（PRSV-W）的南瓜叶片中部分纯化出圆柱形内含体蛋白（CIPs）、无定形内含体蛋白（AIPs）和病毒粒子。制备了针对这些单个抗原（ZYMV和WMV2的AIPs除外）的抗血清，并用于通过电印迹酶联免疫吸附测定法（electroblot-ELISA）测定同源和异源抗原之间的血清学分化指数（SDIs）。ZYMV和WMV2的CIPs之间、ZYMV和PRSV-W的CIPs之间以及WMV2和PRSV-W的CIPs之间的平均SDIs分别为1.5、3.5和2.5。ZYMV和PRSV-W的AIPs之间以及WMV2和PRSV-W的AIPs之间的SDIs分别为5和3。ZYMV和WMV2的衣壳蛋白（CPs）之间、ZYMV和PRSV-W的CPs之间以及WMV2和PRSV-W的CPs之间的平均SDIs分别为3、7和8。这些数据表明，ZYMV和WMV2之间的血清学关系比PRSV-W和ZYMV之间或PRSV-W和WMV2之间的血清学关系更为密切，并且在这三种马铃薯Y病毒中，CIP和AIP的抗原决定簇比CP的抗原决定簇保守性更强。
