Kundu A K, Ohshima K, Sako N, Yaegashi H
Laboratory of Plant Virology, Faculty of Agriculture, Saga University, Japan.
Arch Virol. 2000;145(7):1437-47. doi: 10.1007/s007050070100.
To investigate the antigenic nature of cylindrical inclusion proteins (CIPs) of the potyviruses Turnip mosaic virus (TuMV) and Zucchini yellow mosaic virus (ZYMV), monoclonal antibodies (MAbs) against the two CIPs were produced and epitopes on the CIPs were localized using Escherichia coli-expressed CIP fragments in Western blot analysis. All 23 MAbs against ZYMV CIP reacted only with ZYMV CIP. In contrast out of the 18 MAbs produced against TuMV CIP, 14 MAbs were TuMV CIP-specific while the remaining four MAbs cross-reacted with both CIPs. The four cross-reactive MAbs recognized two distinct epitopes in the N-terminal half of TuMV CIP corresponding to amino acid residues 103-119 and 224-237. Thirteen out of 14 TuMV CIP-specific MAbs recognized two distinct epitopes within residues 1-102 and 120-214, while the other one recognized an epitope within residues 301-644. On the other hand, 21 out of 23 ZYMV CIP-specific MAbs recognized epitopes within residues 1-118, while the remaining two recognized epitopes within residues 301-522. These results suggest that cross-reactive and major virus-specific epitopes are located at the N-terminal half of the respective CIPs.
为研究芜菁花叶病毒（TuMV）和小西葫芦黄花叶病毒（ZYMV）等马铃薯Y病毒属病毒的柱状内含体蛋白（CIPs）的抗原性质，制备了针对这两种CIPs的单克隆抗体（MAbs），并在蛋白质印迹分析中使用大肠杆菌表达的CIP片段对CIPs上的表位进行定位。所有23种针对ZYMV CIP的MAbs仅与ZYMV CIP发生反应。相比之下，在制备的18种针对TuMV CIP的MAbs中，14种MAbs对TuMV CIP具有特异性，而其余4种MAbs与两种CIPs均发生交叉反应。这4种交叉反应性MAbs在TuMV CIP的N端对应于氨基酸残基103 - 119和224 - 237的区域识别出两个不同的表位。14种TuMV CIP特异性MAbs中的13种在残基1 - 102和120 - 214内识别出两个不同的表位，而另一种识别残基301 - 644内的一个表位。另一方面，23种ZYMV CIP特异性MAbs中的21种在残基1 - 118内识别表位，其余两种在残基301 - 522内识别表位。这些结果表明，交叉反应性和主要病毒特异性表位位于各自CIPs的N端区域。
