Department of Animal Health, Veterinary Science School, Complutense University of Madrid, 28040 Madrid, Spain.
Res Vet Sci. 2013 Jun;94(3):817-9. doi: 10.1016/j.rvsc.2013.01.004. Epub 2013 Feb 4.
The aim of the present study was to compare the efficiency of two PCR techniques for the diagnosis of small ruminant lentiviruses (SRLVs). Detection of the proviral genome by PCR, though sensitive, is difficult due to the heterogeneity of the SRLV genomes. One of the PCR techniques amplifies a fragment in the pol gene (pol-PCR) and the other PCR targets the LTR region of the proviral genome (LTR-PCR). Milk from 194 sheep and 163 goats from farms in the Central Spain was analyzed by both techniques and compared to results obtained by ELISA. When compared to the serologic assay, the agreement of both PCR techniques was very low (0.024 and 0.020 in sheep, and 0.124 and 0.114 in goats). In view of these results, it may be concluded that the efficacy of PCR for the diagnosis of SRLVs is low and a combination of PCR and ELISA should be used for diagnosis.
本研究旨在比较两种聚合酶链反应（PCR）技术在诊断小反刍动物慢病毒（SRLV）方面的效率。虽然 PCR 检测病毒前基因组具有敏感性，但由于 SRLV 基因组的异质性，检测变得困难。其中一种 PCR 技术扩增 pol 基因中的一个片段（pol-PCR），另一种 PCR 则针对前病毒基因组的 LTR 区域（LTR-PCR）。通过这两种技术对来自西班牙中部农场的 194 只绵羊和 163 只山羊的牛奶进行了分析，并与 ELISA 检测结果进行了比较。与血清学检测相比，这两种 PCR 技术的一致性非常低（绵羊为 0.024 和 0.020，山羊为 0.124 和 0.114）。鉴于这些结果，可以得出结论，PCR 诊断 SRLV 的效果不佳，应将 PCR 和 ELISA 联合使用进行诊断。
