Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avenida Puerta de Hierro, 28040 Madrid, Spain.
Vet Rec. 2011 Jan 8;168(1):20. doi: 10.1136/vr.c4951. Epub 2011 Jan 4.
The diagnostic performance of an ELISA for the detection of antibodies to the small ruminant lentiviruses (SRLVs) maedi-visna virus and caprine arthritis-encephalitis virus in milk and corresponding blood samples was evaluated in 50 sheep. The agreement between ELISA results in blood and milk was 90 per cent, and the κ value was 0.79. In addition, a serological survey in the central zone of Spain was performed using milk samples from 413 animals (250 sheep and 163 goats) from 12 flocks/herds. All flocks/herds had some animals that were positive for SRLV. Among the animals, 60.0 per cent of the sheep and 8.0 per cent of the goats tested were seropositive. Each sample was also tested using a PCR technique, which increased the percentage of positive animals detected. Using a combination of ELISA and PCR gave a total of 72.2 per cent of sheep and 28.8 per cent of goats positive for SRLV.
我们评估了一种 ELISA 检测试剂盒在牛奶和相应血液样本中检测小反刍动物慢病毒（SRLVs）绵羊肺腺瘤病毒和山羊关节炎-脑炎病毒抗体的诊断性能。在 50 只绵羊中，血液和牛奶中 ELISA 结果的一致性为 90%，κ 值为 0.79。此外，我们还在西班牙中部地区进行了一项血清学调查，检测了来自 12 个羊群/畜群的 413 只动物（250 只绵羊和 163 只山羊）的牛奶样本。所有的羊群/畜群都有一些动物呈 SRLV 阳性。在这些动物中，60.0%的绵羊和 8.0%的山羊检测呈血清阳性。每个样本还使用 PCR 技术进行了检测，这增加了检测到的阳性动物的比例。使用 ELISA 和 PCR 的组合检测，绵羊的阳性率为 72.2%，山羊的阳性率为 28.8%。
