采用高效薄层色谱-光密度法对十六烷基磷胆碱进行定量分析。

Quantitation of hexadecylphosphocholine by high-performance thin-layer chromatography with densitometry.

作者信息

Rustenbeck I, Lenzen S

机构信息

Institute of Pharmacology and Toxicology, University of Göttingen, F.R.G.

出版信息

J Chromatogr. 1990 Jan 26;525(1):85-91. doi: 10.1016/s0378-4347(00)83381-2.

DOI:10.1016/s0378-4347(00)83381-2

PMID:2338450

Abstract

A method for the determination of the antineoplastic ether phospholipid hexadecylphosphocholine (HePC) is presented, based on the separation of the lipids by high-performance thin-layer chromatography charring with a cupric sulphate reagent and quantitation by in situ densitometry. The lower limit of determination is ca. 25 pmol. Concentrated hexane-isopropanol extracts of plasma samples can be applied to the plate without further clean-up, making this method useful for clinical drug monitoring. Additional ion-exchange chromatography and removal of the salt contaminants by gel filtration permits the study of endogenous phospholipids together with HePC from the same sample.

摘要

本文介绍了一种测定抗肿瘤醚磷脂十六烷基磷胆碱（HePC）的方法，该方法基于通过高效薄层色谱法用硫酸铜试剂炭化分离脂质，并通过原位密度测定法定量。测定下限约为25皮摩尔。血浆样品的浓缩己烷 - 异丙醇提取物无需进一步净化即可应用于薄板，使得该方法可用于临床药物监测。额外的离子交换色谱法和通过凝胶过滤去除盐污染物允许对来自同一样品的内源性磷脂和HePC进行研究。