高效薄层色谱-密度法：蔓越莓提取物质量控制的进一步发展。
High performance thin layer chromatography-densitometry: a step further for quality control of cranberry extracts.
机构信息
UMR INRA 1282 Infectiologie et Santé Publique, Equipe Recherche et Innovation en Chimie Médicinale, UFR Sciences Pharmaceutiques, Université de Tours François Rabelais, 31 Avenue Monge, 37200 Tours, France.
出版信息
Food Chem. 2013 Aug 15;139(1-4):866-71. doi: 10.1016/j.foodchem.2013.02.002. Epub 2013 Feb 11.
A method for selective quantitation of catechin, proanthocyanidin (PAC) A2 and PAC-B1 in American cranberry (Vaccinium macrocarpon) extracts using high performance thin layer chromatography (HPTLC)-densitometry is presented. Methylene chloride/ethyl acetate/formic acid (6:10:1, v/v) as the mobile phase and 1% vanillin hydrochloric solution as staining reagent were used. In these conditions, three standards considered as quality markers, catechin, PAC-A2 and PAC-B1, were well resolved allowing simultaneous quantitation on one plate. All standards were quantified in the range of 0.7-5 μg with RSD of repeatability and intermediate precision not exceeding 5%. Catechin, PAC-A2 and PAC-B1 profiles of cranberry extracts were analysed regarding global PAC amounts obtained by BL-DMAC assay. It appears clearly that HPTLC-densitometry process provides additional information which, combined with BL-DMAC results, allows qualitative and quantitative control of cranberry extracts. Particularly, densitometric assay highlighted degradation of PACs in a 7 day-extract, leading to high overestimation with BL-DMAC protocol.
本文介绍了一种使用高效薄层色谱（HPTLC）-密度法选择性定量分析蔓越莓（Vaccinium macrocarpon）提取物中儿茶素、原花青素（PAC）A2 和 PAC-B1 的方法。采用二氯甲烷/乙酸乙酯/甲酸（6:10:1，v/v）作为流动相，1%香草醛盐酸溶液作为显色剂。在这些条件下，三种被认为是质量标志物的标准品，即儿茶素、PAC-A2 和 PAC-B1，得到了很好的分离，允许在同一张板上同时定量。所有标准品在 0.7-5μg 范围内进行定量，重复性和中间精密度的 RSD 不超过 5%。根据 BL-DMAC 测定法获得的总 PAC 量，分析了蔓越莓提取物的儿茶素、PAC-A2 和 PAC-B1 图谱。显然，HPTLC 密度法提供了额外的信息，与 BL-DMAC 结果相结合，可以对蔓越莓提取物进行定性和定量控制。特别是，密度测定法强调了 7 天提取物中 PACs 的降解，导致 BL-DMAC 方案的过高估计。
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