High Altitude Plant Physiology Research Centre, Srinagar, Pauri Garhwal 246 174, Uttarakhand, India.
J Biosci. 2013 Mar;38(1):13-9. doi: 10.1007/s12038-012-9284-5.
Dormancy-breaking and seed germination studies in genus Lilium reveal that the majority of Lilium spp. studied have an underdeveloped embryo at maturity, which grows inside the seed before the radicle emerges. Additionally, the embryo, radicle or cotyledon has a physiological component of dormancy; thus, Lilium seeds have morphophysiological dormancy (MPD). A previous study suggested that seeds of Lilium polyphyllum have MPD but the study did not investigate the development of the embryo, which is one of the main criteria to determine MPD in seeds. To test this hypothesis, we investigated embryo growth and emergence of radicles and epicotyls in seeds over a range of temperatures. At maturity, seeds had underdeveloped embryos which developed fully at warm temperature within 6 weeks. Immediately after embryo growth, radicles also emerged at warm temperatures. However, epicotyls failed to emerge soon after radicle emergence. Epicotyls emerged from >90% seeds with an emerged radicle only after they were subjected to 2 weeks of cold moist stratification. The overall temperature requirements for dormancy-breaking and seed germination indicate a non-deep simple epicotyl MPD in L. polyphyllum.
休眠打破和种子萌发研究表明，大多数研究的百合属植物在成熟时具有未充分发育的胚胎，该胚胎在胚根出现之前在种子内部生长。此外，胚胎、胚根或子叶具有休眠的生理成分；因此，百合属种子具有形态生理休眠（MPD）。先前的一项研究表明，百合 polyphyllum 的种子具有 MPD，但该研究并未调查胚胎的发育，这是确定种子中 MPD 的主要标准之一。为了验证这一假设，我们在一系列温度下研究了种子中胚胎的生长和胚根和上胚轴的出现。在成熟时，种子具有未充分发育的胚胎，在温暖的温度下 6 周内完全发育。胚胎生长后，胚根立即在温暖的温度下出现。然而，上胚轴在胚根出现后不久未能出现。上胚轴在 >90%的种子中出现，只有在经过 2 周的冷湿层积处理后，带有出现的胚根的种子才能出现。休眠打破和种子萌发的总体温度要求表明，百合 polyphyllum 中存在非深简单上胚轴 MPD。
