Sharma Bharti, Kramer Elena M
Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, MA, USA.
Methods Mol Biol. 2013;975:71-81. doi: 10.1007/978-1-62703-278-0_6.
Aquilegia Origami is an emerging model system for ecology and evolution, which has numerous genetic and genomic tools. Virus-induced gene silencing (VIGS) has been established as an effective approach to study gene function in Aquilegia. In the current protocol, we demonstrate VIGS using Agrobacterium strain GV3101 carrying tobacco rattle virus (TRV)-based constructs to infect Aquilegia coerulea "Origami" plants via vacuum infiltration.
耧斗菜“折纸”是一个新兴的生态与进化模型系统，拥有众多遗传和基因组工具。病毒诱导基因沉默（VIGS）已成为研究耧斗菜基因功能的有效方法。在本实验方案中，我们展示了利用携带基于烟草脆裂病毒（TRV）构建体的根癌农杆菌菌株GV3101，通过真空渗透法感染蓝花耧斗菜“折纸”植株来进行VIGS。
