Instituto de Biología, Universidad de Antioquia, Medellín, Colombia.
Departamento de Ciencias Naturales y Matemáticas, Pontificia Universidad Javeriana, Cali, Colombia.
Methods Mol Biol. 2023;2686:365-401. doi: 10.1007/978-1-0716-3299-4_19.
Researchers working on evolutionary developmental plant biology are inclined to choose non-model taxa to address how specific features have been acquired during ontogeny and fixed during phylogeny. In this chapter we describe methods to extract RNA, to assemble de-novo transcriptomes, to isolate orthologous genes within gene families, and to evaluate expression and function of target genes. We have successfully optimized these protocols for non-model plant species including ferns, gymnosperms, and a large assortment of angiosperms. In the latter, we have ranged a large number of families including Aristolochiaceae, Apodanthaceae, Chloranthaceae, Orchidaceae, Papaveraceae, Rubiaceae, Solanaceae, and Tropaeolaceae.
从事进化发育植物生物学研究的人员倾向于选择非模式生物类群来解决特定特征在个体发育中是如何获得的，并在系统发育中是如何固定的。在本章中，我们描述了提取 RNA、从头组装转录组、分离基因家族内的直系同源基因以及评估靶基因表达和功能的方法。我们已经成功地为包括蕨类植物、裸子植物和大量被子植物在内的非模式植物物种优化了这些方案。在后一种情况下，我们研究了许多科，包括马兜铃科、角胡麻科、金粟兰科、兰科、罂粟科、茜草科、茄科和旱金莲科。
