An alternative method for determining inhibition rate constants by following the substrate reaction.

An alternative plotting method is described by which microscopic inhibition rate constants can be determined by following the substrate reaction in the presence of the irreversible inhibitor. Not only does this method keep the advantage of Tsou's original method (Tsou, 1965a. Acta Biochem. biophys. Sin. 5, 1028-1032; 1965b. Acta Biochem. biophys. Sin 5, 409-417), but also is suitable for the cases where the consumption of substrate and accumulation of product must be taken into account.