一种测定抑制常数的快速方法。
A quick method for the determination of inhibition constants.
作者信息
Waley S G
出版信息
Biochem J. 1982 Sep 1;205(3):631-3. doi: 10.1042/bj2050631.
Abstract
The inhibition constant Ki in the common case of competitive inhibition can be obtained by simple comparison of progress curves in the presence and in the absence of inhibitor. The difference between the times taken for the concentration of substrate to fall to the same value is used to obtain Ki. The procedure to use when the product inhibits is described. When there is mixed inhibition, reactions at different substrate concentrations are used to obtain both inhibition constants.
摘要
在竞争性抑制的常见情况下，抑制常数Ki可通过简单比较存在抑制剂和不存在抑制剂时的进程曲线来获得。利用底物浓度降至相同值所需时间的差异来获取Ki。文中描述了产物产生抑制作用时的使用步骤。当存在混合抑制时，利用不同底物浓度下的反应来获得两个抑制常数。
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