Rupp R A, Kruse U, Multhaup G, Göbel U, Beyreuther K, Sippel A E
Zentrum für Molekulare Biologie der Universität Heidelberg, FRG.
Nucleic Acids Res. 1990 May 11;18(9):2607-16. doi: 10.1093/nar/18.9.2607.
Chicken TGGCA proteins belong to the ubiquitous, eukaryotic family of NFI-like nuclear proteins, which share an identical DNA binding specificity. They are involved in viral and cellular aspects of transcriptional regulation and they are capable of stimulating Adenovirus initiation of replication. Using microsequencing data from peptides of isolated proteins and PCR supported cloning, we have derived four cDNAs for NFI/TGGCA proteins, which are encoded by three separate chicken genes. Sequence alignments of NFI proteins from chicken and various mammalian species provide evidence for a common genetic equipment among higher eukaryotes, in which several related genes, employing each differential RNA splicing generate an unexpectedly large family of diverse NFI proteins. The extensive similarity of the amino acid sequence throughout the complete coding regions between products of the same gene type in different species indicates a uniform selection pressure on all protein parts, also on those outside the DNA-binding domain.
鸡的TGGCA蛋白属于普遍存在的真核生物类NFI样核蛋白家族，它们具有相同的DNA结合特异性。它们参与转录调控的病毒和细胞方面，并且能够刺激腺病毒的复制起始。利用从分离蛋白的肽段获得的微测序数据以及PCR支持的克隆技术，我们得到了四个NFI/TGGCA蛋白的cDNA，它们由三个不同的鸡基因编码。鸡和各种哺乳动物物种的NFI蛋白的序列比对为高等真核生物之间的共同遗传组成提供了证据，其中几个相关基因通过各自不同的RNA剪接产生了一个意外庞大的多样化NFI蛋白家族。不同物种中相同基因类型产物在整个完整编码区域的氨基酸序列的广泛相似性表明对所有蛋白质部分，包括DNA结合结构域之外的部分，都存在统一的选择压力。
