Chang Yung-Ming, Tsai Chuan-Te, Wang Chiun-Chuang Roger, Chen Yueh-Sheng, Lin Yueh-Min, Kuo Chia-Hua, Tzang Bor-Show, Chen Ray-Jade, Tsai Fuu-Jen, Huang Chih-Yang
Department of Chinese Medicine, China Medical University Hospital, Taichung, Taiwan.
Biosci Biotechnol Biochem. 2013;77(2):229-34. doi: 10.1271/bbb.120541. Epub 2013 Feb 7.
Angiotensin II (Ang II) is a risk factor for cardiovascular disease. We used a traditional Chinese medicine, alpinate oxyphyllae fructus (AOF), to evaluate its effect on Ang II-induced cardiac apoptosis and mitochondrial dysfunction. Ang II-treated H9c2 cells were administered AOF of 20-100 µg/mL concentrations. Ang II significantly increased TUNEL-positive nuclei in the H9c2 cells, effect was inhibited by AOF administration in both pre-treated and post-treated H9c2 cells. Caspases 9 and 3 activities were increased by Ang II and downregulated by AOF administration, especially in pre-treatment. AOF treatment reversed Ang II-induced mitochondria membrane potential instability in H9c2 cells as observed by JC-1 stain assay. Furthermore, pro-apoptotic proteins Bad and cytochrome c increased and decreased respectively under AOF administration. The levels of p-Bad anti-apoptotic protein were significantly increased after AOF treatment. This study indicates that mitochondrial dependent apoptosis induced by Ang II.
血管紧张素II（Ang II）是心血管疾病的一个风险因素。我们使用一种传统中药益智仁（AOF）来评估其对Ang II诱导的心脏细胞凋亡和线粒体功能障碍的影响。用浓度为20 - 100μg/mL的AOF处理经Ang II处理的H9c2细胞。Ang II显著增加了H9c2细胞中TUNEL阳性细胞核，在预处理和后处理的H9c2细胞中，AOF给药均抑制了该效应。Ang II增加了半胱天冬酶9和3的活性，而AOF给药下调了其活性，尤其是在预处理时。通过JC-1染色分析观察到，AOF处理逆转了Ang II诱导的H9c2细胞线粒体膜电位不稳定。此外，在AOF给药后，促凋亡蛋白Bad增加而细胞色素c减少。AOF处理后，抗凋亡蛋白p-Bad的水平显著增加。本研究表明Ang II诱导线粒体依赖性凋亡。
