Larsson H, Persson S U, Hedner P
Department of Internal Medicine, University Hospital, Lund, Sweden.
Scand J Clin Lab Invest. 1990 Apr;50(2):177-81. doi: 10.1080/00365519009089150.
Blood samples from nine healthy men were studied to determine the effect of ouabain and elevated serum calcium concentration on blood viscosity, measured by a rotational viscometer, and on red cell filterability by the St George's Filtrometer, giving values for clogging particles (CP) and red cell transit time (RCTT). Blood viscosity at a standardized haematocrit of 45% and red cell filterability was investigated in blood samples incubated for 1 h with Ringer's solution only (control), with ouabain (0.70 mmol/l) in plasma, or with serum calcium concentration increased by 3.0 mmol/l by addition of CaCl2. Incubation with ouabain significantly reduced erythrocyte K+ concentration and increased that of Na+. Ouabain caused a decrease in blood viscosity (p less than 0.05-0.005) compared to controls, although there was no decrease in red cell filterability parameters. When incubating with calcium, CP and RCTT increased significantly indicating 'stiffer' red cells, but there was no increase in blood viscosity. It is concluded that blood viscosity may be influenced by red cell factors not detected by CP or RCTT, which in turn appear to reflect red cell deformability with greater sensitivity and specificity than blood viscosity. It is concluded also that the functional state of the cell membrane may be of significance for the rheological properties of erythrocytes.
对9名健康男性的血液样本进行了研究，以确定哇巴因和血清钙浓度升高对血液粘度（通过旋转粘度计测量）以及对红细胞过滤性（通过圣乔治滤过仪测量，得出阻塞颗粒（CP）和红细胞通过时间（RCTT）的值）的影响。在仅用林格氏液孵育1小时的血液样本（对照）、血浆中含有哇巴因（0.70 mmol/l）的血液样本或通过添加CaCl2使血清钙浓度升高3.0 mmol/l的血液样本中，研究了标准化血细胞比容为45%时的血液粘度和红细胞过滤性。用哇巴因孵育可显著降低红细胞K+浓度并升高Na+浓度。与对照组相比，哇巴因导致血液粘度降低（p小于0.05 - 0.005），尽管红细胞过滤性参数没有降低。当与钙孵育时，CP和RCTT显著增加，表明红细胞“更硬”，但血液粘度没有增加。得出的结论是，血液粘度可能受CP或RCTT未检测到的红细胞因素影响，而CP和RCTT反过来似乎比血液粘度更敏感、更特异地反映红细胞变形性。还得出结论，细胞膜的功能状态可能对红细胞的流变学特性具有重要意义。
