Enomoto M, Tanimizu I, Sakuragawa N
Research Laboratories, Nippon Shoji Kaisha, Ltd., Osaka, Japan.
Thromb Res. 1990 Mar 1;57(5):729-36. doi: 10.1016/0049-3848(90)90030-g.
A new biological method for the assay of plasma antithrombin-III (AT-III) activity was developed without influence from heparin cofactor II (HC-II). AT-III deficient plasma is used as a substrate and diluted prothrombin time reagent as a reaction trigger for the specific assay of AT-III. The AT-III deficient plasma is prepared by passage of plasma through a heparin-agarose column. In the presence of heparin, AT-III in the sample shows concentration dependent anticoagulant activity and calibration curve is linear on semi-logarithmic graph paper. The results of reproducibility, recoveries and correlation studies with a chromogenic assay indicate that this biological method is reliable and suitable for routine use in clinical laboratories. Influence of HC-II is minimal. The method provides several advantages over those of chromogenic substrate and fibrinogen.
一种用于测定血浆抗凝血酶III（AT-III）活性的新生物学方法被开发出来，该方法不受肝素辅因子II（HC-II）的影响。将缺乏AT-III的血浆用作底物，并将稀释的凝血酶原时间试剂用作AT-III特异性测定的反应触发剂。通过使血浆通过肝素琼脂糖柱来制备缺乏AT-III的血浆。在肝素存在的情况下，样品中的AT-III表现出浓度依赖性抗凝活性，并且校准曲线在半对数坐标纸上呈线性。与发色底物法相比，该方法的重现性、回收率及相关性研究结果表明，这种生物学方法可靠且适用于临床实验室的常规使用。HC-II的影响极小。该方法比发色底物法和纤维蛋白原法具有多个优势。
