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本文引用的文献

1
A competitive immunoassay with a surrogate calibrator curve for aflatoxin M1 in milk.牛奶中黄曲霉毒素 M1 的竞争免疫分析及其替代校准曲线。
Anal Chim Acta. 2011 Oct 3;703(1):64-9. doi: 10.1016/j.aca.2011.07.011. Epub 2011 Jul 19.
2
Production of ultrasensitive generic monoclonal antibodies against major aflatoxins using a modified two-step screening procedure.采用改良的两步筛选程序生产针对主要黄曲霉毒素的超灵敏通用单克隆抗体。
Anal Chim Acta. 2009 Mar 16;636(1):63-9. doi: 10.1016/j.aca.2009.01.010. Epub 2009 Jan 10.
3
Phage-borne peptidomimetics accelerate the development of polyclonal antibody-based heterologous immunoassays for the detection of pesticide metabolites.噬菌体携带的拟肽可加速基于多克隆抗体的异源免疫分析方法的开发，用于检测农药代谢物。
Environ Sci Technol. 2008 Mar 15;42(6):2047-53. doi: 10.1021/es702219a.
4
Analyte peptidomimetics selected from phage display peptide libraries: a systematic strategy for the development of environmental immunoassays.从噬菌体展示肽库中筛选的分析物拟肽：环境免疫分析开发的系统策略
Environ Sci Technol. 2005 Jun 1;39(11):4234-41. doi: 10.1021/es047931l.
5
Development of an immunoassay-based lateral flow dipstick for the rapid detection of aflatoxin B1 in pig feed.一种基于免疫分析的侧流试纸条用于快速检测猪饲料中黄曲霉毒素B1的研发。
J Agric Food Chem. 2005 May 4;53(9):3364-8. doi: 10.1021/jf0404804.
6
A rapid aflatoxin B1 ELISA: development and validation with reduced matrix effects for peanuts, corn, pistachio, and Soybeans.一种快速黄曲霉毒素B1酶联免疫吸附测定法：针对花生、玉米、开心果和大豆开发并验证，减少基质效应
J Agric Food Chem. 2004 May 19;52(10):2746-55. doi: 10.1021/jf0354038.
7
Limited survey for the presence of aflatoxins in foods from local markets and supermarkets in Valencia, Spain.西班牙巴伦西亚当地市场和超市食品中黄曲霉毒素存在情况的有限调查。
Food Addit Contam. 2004 Feb;21(2):165-71. doi: 10.1080/0265203031000.
8
Update of survey, regulation and toxic effects of mycotoxins in Europe.欧洲霉菌毒素调查、监管及毒性效应的最新情况
Toxicol Lett. 2002 Feb 28;127(1-3):19-28. doi: 10.1016/s0378-4274(01)00479-9.
9
Phage-displayed peptides that mimic aflatoxin B1 in serological reactivity.在血清学反应中模拟黄曲霉毒素B1的噬菌体展示肽。
J Appl Microbiol. 2001 Mar;90(3):330-6. doi: 10.1046/j.1365-2672.2001.01249.x.
10
Production and characterization of a monoclonal anti-anti-idiotype antibody against fumonisin B(1).抗伏马菌素B(1)单克隆抗独特型抗体的制备与鉴定
J Agric Food Chem. 1999 Nov;47(11):4815-20. doi: 10.1021/jf990185x.

展示类病毒展示肽模拟黄曲霉毒素及其在免疫测定中的应用。

Phage-displayed peptide that mimics aflatoxins and its application in immunoassay.

机构信息

Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences , Wuhan 430062, P. R. China.

出版信息

J Agric Food Chem. 2013 Mar 13;61(10):2426-33. doi: 10.1021/jf4004048. Epub 2013 Feb 27.

DOI:10.1021/jf4004048
PMID:23394544
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3830680/
Abstract

To search for an alternative to using protein conjugated aflatoxin as a coating antigen in aflatoxin detection by an ELISA method, a random-8-peptide library was constructed and used as a source of peptides that mimic aflatoxins (termed as mimotopes). Five mimotope peptides were obtained by panning-elution from the library and were successfully used in an indirect competitive ELISA for analyzing total aflatoxin concentration. The assay exhibited an IC50 value of 14 μg/kg in samples (with 1 in 7 dilution of sample extract) for aflatoxins. The linear range is 4-24 μg/kg. Further validation indicated relatively good recovery (60-120%) in peanut, rice and corn. Natural contaminated samples (peanut and feedstuff) were analyzed for aflatoxin concentration by both conventional ELISA and phage ELISA. The results showed good correlation. It can be concluded that the mimotope preparation is an effective substitute for the aflatoxin based coating antigen in ELISA and can be used in real sample analysis.

摘要

为了寻找替代物来替代 ELISA 方法中使用的蛋白结合黄曲霉毒素作为包被抗原，构建了一个随机 8 肽库，并将其用作模拟黄曲霉毒素的肽的来源（称为模拟表位）。通过从文库中进行淘选-洗脱，获得了 5 个模拟表位肽，并成功地用于间接竞争 ELISA 分析总黄曲霉毒素浓度。该测定法在样品中的 IC50 值为 14 μg/kg（样品提取物稀释 1/7）。线性范围为 4-24 μg/kg。进一步的验证表明，在花生、大米和玉米中的回收率相对较好（60-120%）。通过常规 ELISA 和噬菌体 ELISA 对天然污染样品（花生和饲料）进行了黄曲霉毒素浓度分析。结果表明相关性良好。可以得出结论，模拟表位制备是 ELISA 中基于黄曲霉毒素的包被抗原的有效替代品，可用于实际样品分析。