Kohri K, Uemura T, Iguchi M, Kurita T
Department of Urology, Kinki University, School of Medicine, Osaka, Japan.
Urol Res. 1990;18(2):101-5. doi: 10.1007/BF00302468.
Exposure of bladder tumor cell strain HT-1197, chronic bonemarrow leukemic cell strain K-562, and African green-turtle normal kidney cell strain Vero to high energy shock waves resulted in ultrastructural changes and a reduction in cell viability as determined by 3H-thymidine incorporation assay and flowcytometer. K-562 was the most sensitive while Vero was the most resistant to the high energy shock wave. By flowcytometry using anti BrdU antibody, described K-562 in the S phase was found to be inhibited by the exposure. Electron microscopy revealed destruction of microvilli over the cell surface and swollen mitochondria in K-562 and HT-1197. These effects were related to the number of high energy shock wave exposures. Our study demonstrates that a high energy shock wave has an anti-tumor effect in vitro.
将膀胱肿瘤细胞株HT - 1197、慢性骨髓白血病细胞株K - 562和非洲绿海龟正常肾细胞株Vero暴露于高能冲击波下，会导致超微结构改变以及细胞活力降低，这是通过³H - 胸腺嘧啶核苷掺入试验和流式细胞仪测定得出的结果。K - 562对高能冲击波最为敏感，而Vero最具抗性。通过使用抗BrdU抗体的流式细胞术发现，处于S期的K - 562细胞受到暴露的抑制。电子显微镜检查显示，K - 562和HT - 1197细胞表面的微绒毛遭到破坏，线粒体肿胀。这些效应与高能冲击波的暴露次数有关。我们的研究表明，高能冲击波在体外具有抗肿瘤作用。
