Research Laboratory for the Physical and Chemical Testing of Foods, Department of Food Technology, School of Food Technology and Nutrition, Alexander Technological Educational Institute (ATEI) of Thessaloniki, GR-57400, Thessaloniki, Greece.
J Chromatogr B Analyt Technol Biomed Life Sci. 2013 May 15;927:218-22. doi: 10.1016/j.jchromb.2013.01.011. Epub 2013 Jan 20.
An ultra high pressure liquid chromatographic method coupled with diode array detector (UHPLC-DAD) has been developed and validated for the fast separation and determination of three major methylxanthines, i.e., caffeine, theophylline and theobromine, in various herbal beverages. Isocratic elution using 0.1vol% formic acid/CH3OH (92.5:7.5, v/v) enabled the completion of the separation cycle in less than 3min using a flow rate of 0.7mL/min and a column temperature of 50°C. Validation of the method included linearity (0.5-50mg/L), limits of detection (12-35μg/L) and quantification (40-120μg/L), precision, matrix effect and accuracy. The percent recoveries ranged between 90 and 108%.
建立并验证了一种超高效液相色谱-二极管阵列检测（UHPLC-DAD）法，用于快速分离和测定各种草本饮料中的三种主要甲基黄嘌呤：咖啡因、茶碱和可可碱。采用 0.1vol%甲酸/甲醇（92.5:7.5，v/v）等度洗脱，流速为 0.7mL/min，柱温为 50°C，不到 3min 即可完成分离周期。方法验证包括线性（0.5-50mg/L）、检测限（12-35μg/L）和定量限（40-120μg/L）、精密度、基质效应和准确度。回收率在 90%至 108%之间。
