Kopp J B, Robey P G
Bone Research Branch, NIDR, NIH, Bethesda, Maryland.
J Bone Miner Res. 1990 Mar;5 Suppl 1:S137-41. doi: 10.1002/jbmr.5650051321.
Sodium fluoride has been shown to be effective therapy for some patients with vertebral osteoporosis. Data from histomorphometric studies in patients and animals suggest that at least part of this effect may be a consequence of a proliferative effect of fluoride, either direct or indirect, on the osteoblast or on an osteoblastic precursor cell. Experiments with osteoblastic cells derived from embryonic chick calvaria have demonstrated a mitogenic effect of fluoride. The present study examined whether fluoride affects in a similar way fetal human bone cells derived from femur or calvaria. Under a variety of culture conditions, including medium supplemented with serum and in serum-free medium, fluoride did not alter the proliferative rate of human bone cells as measured by thymidine incorporation and direct cell counting.
氟化钠已被证明对一些椎体骨质疏松症患者是有效的治疗方法。来自患者和动物的组织形态计量学研究数据表明，这种效应至少部分可能是氟化物对成骨细胞或成骨前体细胞直接或间接的增殖作用的结果。对来自胚胎鸡颅骨的成骨细胞进行的实验已证明氟化物具有促有丝分裂作用。本研究检测了氟化物是否以类似方式影响源自股骨或颅骨的胎儿人骨细胞。在多种培养条件下，包括添加血清的培养基和无血清培养基中，通过胸腺嘧啶核苷掺入和直接细胞计数测量，氟化物并未改变人骨细胞的增殖速率。
