Zorlu Fatma Yalcin, Darici Hakan, Turkkahraman Hakan
Department of Orthodontics, School of Dentistry, University of Suleyman Demirel, Isparta, Turkey.
Department of Histology & Embryology, School of Medicine, University of Suleyman Demirel, Isparta, Turkey.
Eur J Dent. 2019 Jul;13(3):361-369. doi: 10.1055/s-0039-1700192. Epub 2019 Dec 3.
The aim of this study was to determine the effects of systemic fluoride intake on orthodontic tooth movement with histomorphometric and histopathologic methods.
Forty-eight Wistar albino rats were randomly divided into four groups of 12 rats each. Group I received fluoridated water and underwent orthodontic tooth movement. Group II received fluoridated water and did not undergo orthodontic tooth movement. Group III received nonfluoridated water and underwent orthodontic tooth movement. Group IV received nonfluoridated water and did not undergo orthodontic tooth movement. At the beginning of the experiment (T1), impressions were taken from the maxilla of the rats in groups I and III under general anesthesia, and a NiTi closed coil spring appliance was ligated between the left maxillary central incisors and maxillary first molar. The orthodontic force applied was approximately 75 g, and the duration of the experimental period was 18 days. During the experimental period, appliances were controlled daily. At the end of the experimental period (T2), the rats were sacrificed with an overdose of a ketamine/xylasine combination, and their impressions were obtained. The upper first molars were subsequently dissected for histological examination. Incisor-molar distance, number of osteoblasts, number of osteoclasts and periodontal ligament (PDL) space widths on the compression and tension sides were measured.
All measurements were statistically analyzed with SPSS for Windows version 18.0 (SPSS Inc., Chicago, IL, USA). Repeated measures ANOVA and posthoc Tukey tests were used to compare the groups.
No statistically significant difference was found with respect to the amount of tooth movement between the fluoridated and nonfluoridated groups ( > 0.05). Orthodontic force application increased the number of osteoblasts at the tension sides and reduced it at the compression sides ( < 0.001). An increased number of osteoclasts was observed in the nonfluoridated group relative to the fluoridated group ( < 0.01).
No difference was observed with respect to the amount of tooth movement between the fluoridated and nonfluoridated groups. Fluoride significantly reduced the number of osteoclasts in the experimental groups.
本研究旨在通过组织形态计量学和组织病理学方法确定全身摄入氟化物对正畸牙齿移动的影响。
48只Wistar白化大鼠随机分为四组，每组12只。第一组饮用含氟水并进行正畸牙齿移动。第二组饮用含氟水但未进行正畸牙齿移动。第三组饮用无氟水并进行正畸牙齿移动。第四组饮用无氟水且未进行正畸牙齿移动。在实验开始时（T1），对第一组和第三组大鼠在全身麻醉下取上颌印模，并在上颌左侧中切牙和上颌第一磨牙之间结扎镍钛闭合线圈弹簧矫治器。施加的正畸力约为75克，实验期为18天。实验期间，每天检查矫治器。在实验期结束时（T2），用过量的氯胺酮/赛拉嗪组合处死大鼠，并获取其印模。随后解剖上颌第一磨牙进行组织学检查。测量切牙-磨牙距离、成骨细胞数量、破骨细胞数量以及压缩侧和拉伸侧的牙周膜（PDL）间隙宽度。
所有测量数据使用SPSS for Windows 18.0版软件（美国伊利诺伊州芝加哥市SPSS公司）进行统计学分析。采用重复测量方差分析和事后Tukey检验对各组进行比较。
含氟组和无氟组之间在牙齿移动量方面未发现统计学上的显著差异（>0.05）。施加正畸力使拉伸侧的成骨细胞数量增加，压缩侧的成骨细胞数量减少（<0.001）。相对于含氟组，无氟组观察到破骨细胞数量增加（<0.01）。
含氟组和无氟组在牙齿移动量方面未观察到差异。氟化物显著减少了实验组中的破骨细胞数量。
