Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
NITE Biological Resource Center, Department of Biotechnology (NBRC), National Institute of Technology and Evaluation (NITE), Chiba, Japan.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1568-1573. doi: 10.1099/ijs.0.050013-0. Epub 2013 Feb 8.
Strain DMKU-RK359(T), representing a novel yeast species, was isolated from the external surface of a sugar-cane leaf collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, sequence analysis of the D1/D2 region of the large-subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, strain DMKU-RK359(T) was assigned to a novel Wickerhamomyces species. The novel species was closest to Wickerhamomyces ciferrii, but differed from it by 0.7 % nucleotide substitutions in the D1/D2 region of the LSU rRNA gene and 6 % nucleotide substitutions in the ITS region. The name Wickerhamomyces siamensis sp. nov. is proposed (type strain DMKU-RK359(T) = BCC 50732(T) = NBRC 108900(T) = CBS 12570(T)).
DMKU-RK359(T) 菌株,代表一个新型酵母种,从泰国采集的甘蔗叶外部表面分离得到。基于形态学、生物化学、生理学和化学分类学特征、大亚基(LSU)rRNA 基因 D1/D2 区和内部转录间隔区(ITS)区的序列分析,菌株 DMKU-RK359(T) 被分配到一个新型威克汉姆酵母种。该新型种与威克汉姆酵母最接近,但在 LSU rRNA 基因 D1/D2 区的核苷酸替换率上与它相差 0.7%,在 ITS 区的核苷酸替换率上相差 6%。提议使用新型威克汉姆酵母 siamensis sp. nov. 这一名称(模式菌株 DMKU-RK359(T) = BCC 50732(T) = NBRC 108900(T) = CBS 12570(T))。